PMID- 17542506
OWN - NLM
STAT- MEDLINE
DCOM- 20070628
LR  - 20070604
IS  - 1547-5654 (Print)
IS  - 1547-5646 (Linking)
VI  - 6
IP  - 5
DP  - 2007 May
TI  - Bone marrow stromal cells promoting corticospinal axon growth through the release 
      of humoral factors in organotypic cocultures in neonatal rats.
PG  - 412-9
AB  - OBJECT: The transplantation of bone marrow stromal cells (BMSCs) is considered to 
      be an alternative treatment to promote central nervous system regeneration, but 
      the precise mechanisms of this regeneration after transplantation of BMSCs have 
      not been clarified. In the present study, the authors assessed the effects of 
      BMSC transplantation on corticospinal axon growth quantitatively, and they 
      analyzed the mechanism of central nervous system regeneration in the injured and 
      BMSC-treated spinal cord using the organotypic coculture system. METHODS: Bone 
      marrow stromal cells derived from green fluorescent protein-expressing transgenic 
      Sprague-Dawley rats were transplanted to the organotypic coculture system in 
      which brain cortex and spinal cord specimens obtained in neonatal Sprague-Dawley 
      rats were used. The axon growth from the cortex to the spinal cord was assessed 
      quantitatively, using anterograde tracing with 1,1 
      '-ioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate. To identify the 
      differentiation of transplanted BMSCs, immunohistochemical examinations were 
      performed. In addition, BMSCs were analyzed using reverse transcriptase 
      polymerase chain reaction (RT-PCR) for mRNA expression of the growth factors. The 
      transplantation of BMSCs beneath the membrane, where the transplanted cells did 
      not come into direct contact with the cultured tissue, promoted corticospinal 
      axon growth to the same extent as transplantation of BMSCs on the tissues. The 
      RT-PCR showed that the transplanted BMSCs expressed the mRNA of brain-derived 
      neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF). Con 
      CONCLUSIONS: ese findings strongly suggest that humoral factors expressed by 
      BMSCs, including BDNF and VEGF, participate in regeneration of the central 
      nervous system after transplantation of these cells.
FAU - Kamei, Naosuke
AU  - Kamei N
AD  - Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, 
      Hiroshima University Japan. nahkamei@ybb.ne.jp
FAU - Tanaka, Nobuhiro
AU  - Tanaka N
FAU - Oishi, Yosuke
AU  - Oishi Y
FAU - Ishikawa, Masakazu
AU  - Ishikawa M
FAU - Hamasaki, Takahiko
AU  - Hamasaki T
FAU - Nishida, Koji
AU  - Nishida K
FAU - Nakanishi, Kazuyoshi
AU  - Nakanishi K
FAU - Sakai, Norio
AU  - Sakai N
FAU - Ochi, Mitsuo
AU  - Ochi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosurg Spine
JT  - Journal of neurosurgery. Spine
JID - 101223545
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/*physiology
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Bone Marrow Transplantation
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Ciliary Neurotrophic Factor/metabolism
MH  - Coculture Techniques
MH  - Immunohistochemistry
MH  - Nerve Regeneration/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Cord/*cytology/metabolism
MH  - Stromal Cells/*cytology/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2007/06/05 09:00
MHDA- 2007/06/29 09:00
CRDT- 2007/06/05 09:00
PHST- 2007/06/05 09:00 [pubmed]
PHST- 2007/06/29 09:00 [medline]
PHST- 2007/06/05 09:00 [entrez]
AID - 10.3171/spi.2007.6.5.412 [doi]
PST - ppublish
SO  - J Neurosurg Spine. 2007 May;6(5):412-9. doi: 10.3171/spi.2007.6.5.412.