PMID- 17543235
OWN - NLM
STAT- MEDLINE
DCOM- 20070711
LR  - 20131121
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 53
IP  - 5
DP  - 2007 May 30
TI  - Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients 
      with and without nephropathy.
PG  - 68-78
AB  - Diabetes mellitus is associated with hyperglycemia, hyperlipoproteinaemia and 
      oxidative stress at cellular level. The aim of the present study was to determine 
      the relationship between anti-oxidative system of cells and hyperglycemia induced 
      reactive oxygen species (ROS) in type-1 and type-2 diabetic patients with and 
      without nephropathy. ROS are anihilated by an intracellular enzymatic system such 
      as glutathione peroxidase (GPx) and catalase and the reduced glutathione (GSH) is 
      the major antioxidant of erythrocytes and plasma. Total of 200 diabetic patients 
      and 50 of normal healthy control individuals were recruited for the present 
      study. Erythrocytes and plasma activities of GPx, catalase and GSH were measured 
      as a part of anti-oxidative system and TBARS for the marker of oxidative stress 
      in type-1 diabetes mellitus (T1DM), type-2 diabetic mellitus (T2DM), type-1 
      diabetes with nephropathy (T1DN) and type-2 diabetes with nephropathy (T2DN) 
      patients. GPx, catalase and GSH activity was significantly decreased, whereas 
      TBARS level was significantly increased in both type-1 and type-2 diabetic groups 
      with and without nephropathy than the normal healthy individuals. T1DM patients 
      had shown decreased level of GSH and activity of anti-oxidative enzymes than T2 
      DM patients, whereas TBARS level was higher in T1DM than T2DM patients, 
      suggesting that T1DM patients are more prone to secondary complications such as 
      nephropathy than T2DM patients. Obtained results have shown positive correlation 
      of GSH with GPx and catalase activity in erythrocytes and plasma, whereas, 
      negative correlation was observed between TBARS and catalase in erythrocytes and 
      plasma indicative of the relationship of various anti-oxidative enzymes and key 
      antioxidant such as GSH in erythrocytes and plasma responsible for increased 
      oxidative stress.
FAU - Dave, G S
AU  - Dave GS
AD  - Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, 
      India.
FAU - Kalia, K
AU  - Kalia K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070530
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Catalase/blood
MH  - Diabetes Mellitus, Type 1/*blood/pathology
MH  - Diabetes Mellitus, Type 2/*blood/pathology
MH  - Diabetic Neuropathies/*blood/pathology
MH  - Erythrocytes/metabolism
MH  - Female
MH  - Glutathione/blood
MH  - Glutathione Peroxidase/blood
MH  - Humans
MH  - Hyperglycemia/*blood/complications
MH  - Male
MH  - Middle Aged
MH  - *Oxidative Stress
MH  - Reactive Oxygen Species/blood
MH  - Regression Analysis
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2007/06/05 09:00
MHDA- 2007/07/12 09:00
CRDT- 2007/06/05 09:00
PHST- 2006/10/06 00:00 [received]
PHST- 2007/02/02 00:00 [accepted]
PHST- 2007/06/05 09:00 [pubmed]
PHST- 2007/07/12 09:00 [medline]
PHST- 2007/06/05 09:00 [entrez]
AID - 68 [pii]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2007 May 30;53(5):68-78.