PMID- 17545212
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20161124
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 99
IP  - 1
DP  - 2007 Sep
TI  - Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in the developing male 
      Wistar(Han) rat. I: No decrease in epididymal sperm count after a single acute 
      dose.
PG  - 214-23
AB  - It has been reported that fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin 
      (TCDD) causes defects in the male reproductive system of the rat. We set out to 
      replicate and extend these effects using a robust experimental design. Groups of 
      75 (control vehicle) or 55 (50, 200, or 1000 ng of TCDD/kg bodyweight) female 
      Wistar(Han) rats were exposed to TCDD on gestational day (GD)15, then allowed to 
      litter. The high-dose group dams showed no sustained weight loss compared to 
      control, but four animals had total litter loss. Pups in the high-dose group 
      showed reduced body weight up till day 21, and pups in the medium dose group 
      showed reduced body weight in the first week postpartum. Balano-preputial 
      separation was significantly delayed in the high-dose group male offspring. There 
      were no significant effects of treatment when the offspring were subjected to a 
      functional observational battery or mated with females to assess reproductive 
      capability. Twenty-five males per group were killed on postnatal day (PND) 70, 
      and approximately 60 animals per group (approximately 30 for the high-dose group) 
      on PND120 to assess seminology and other end points. At PND120, the two highest 
      dose groups showed a statistically significant elevation of sperm counts, 
      compared to control; however, this effect was small (approximately 30%), within 
      the normal range of sperm counts for this strain of rat, was not reflected in 
      testicular spermatid counts nor PND70 data, and is therefore postulated to have 
      no biological significance. Although there was an increase in the proportion of 
      abnormal sperm at PND70, seminology parameters were otherwise unremarkable. 
      Testis weights in the high-dose group were slightly decreased at PND70 and 120, 
      and at PND120, brain weights were decreased in the high-dose group, liver to body 
      weight ratios were increased for all three dose groups, with an increase in 
      inflammatory cell foci in the epididymis in the high-dose group. These data show 
      that TCDD is a potent developmental toxin after exposure of the developing fetus 
      but that acute developmental exposure to TCDD on GD15 caused no decrease in sperm 
      counts.
FAU - Bell, David R
AU  - Bell DR
AD  - School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, 
      UK. david.bell@nottingham.ac.uk
FAU - Clode, Sally
AU  - Clode S
FAU - Fan, Ming Qi
AU  - Fan MQ
FAU - Fernandes, Alwyn
AU  - Fernandes A
FAU - Foster, Paul M D
AU  - Foster PM
FAU - Jiang, Tao
AU  - Jiang T
FAU - Loizou, George
AU  - Loizou G
FAU - MacNicoll, Alan
AU  - MacNicoll A
FAU - Miller, Brian G
AU  - Miller BG
FAU - Rose, Martin
AU  - Rose M
FAU - Tran, Lang
AU  - Tran L
FAU - White, Shaun
AU  - White S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070601
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Animals, Newborn
MH  - Behavior, Animal/drug effects
MH  - Body Weight/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Environmental Pollutants/*toxicity
MH  - Epididymis/*drug effects/pathology
MH  - Female
MH  - Fertility/drug effects
MH  - Male
MH  - Maternal Exposure/*adverse effects
MH  - Motor Activity/drug effects
MH  - Organ Size/drug effects
MH  - Perineum/growth & development
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Sexual Maturation/drug effects
MH  - Sperm Count
MH  - Spermatozoa/*drug effects/pathology
MH  - Testis/drug effects/pathology
MH  - *Toxicity Tests, Acute
EDAT- 2007/06/05 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/06/05 09:00
PHST- 2007/06/05 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/06/05 09:00 [entrez]
AID - kfm140 [pii]
AID - 10.1093/toxsci/kfm140 [doi]
PST - ppublish
SO  - Toxicol Sci. 2007 Sep;99(1):214-23. doi: 10.1093/toxsci/kfm140. Epub 2007 Jun 1.