PMID- 17546014
OWN - NLM
STAT- MEDLINE
DCOM- 20080102
LR  - 20080324
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 2
IP  - 5
DP  - 2007
TI  - Imaging mass spectrometry at cellular length scales.
PG  - 1185-96
AB  - Imaging mass spectrometry (IMS) allows the direct investigation of both the 
      identity and the spatial distribution of the molecular content directly in tissue 
      sections, single cells and many other biological surfaces. In this protocol, we 
      present the steps required to retrieve the molecular information from tissue 
      sections using matrix-enhanced (ME) and metal-assisted (MetA) secondary ion mass 
      spectrometry (SIMS) as well as matrix-assisted laser desorption/ionization 
      (MALDI) IMS. These techniques require specific sample preparation steps directed 
      at optimal signal intensity with minimal redistribution or modification of the 
      sample analytes. After careful sample preparation, different IMS methods offer a 
      unique discovery tool in, for example, the investigation of (i) drug transport 
      and uptake, (ii) biological processing steps and (iii) biomarker distributions. 
      To extract the relevant information from the huge datasets produced by IMS, new 
      bioinformatics approaches have been developed. The duration of the protocol is 
      highly dependent on sample size and technique used, but on average takes 
      approximately 5 h.
FAU - Altelaar, A F Maarten
AU  - Altelaar AF
AD  - FOM Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, 
      The Netherlands.
FAU - Luxembourg, Stefan L
AU  - Luxembourg SL
FAU - McDonnell, Liam A
AU  - McDonnell LA
FAU - Piersma, Sander R
AU  - Piersma SR
FAU - Heeren, Ron M A
AU  - Heeren RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (Proteins)
SB  - IM
MH  - Computational Biology/*methods
MH  - Diagnostic Imaging/instrumentation/*methods
MH  - Proteins/*analysis
MH  - Proteomics/instrumentation/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2007/06/05 09:00
MHDA- 2008/01/03 09:00
CRDT- 2007/06/05 09:00
PHST- 2007/06/05 09:00 [pubmed]
PHST- 2008/01/03 09:00 [medline]
PHST- 2007/06/05 09:00 [entrez]
AID - nprot.2007.117 [pii]
AID - 10.1038/nprot.2007.117 [doi]
PST - ppublish
SO  - Nat Protoc. 2007;2(5):1185-96. doi: 10.1038/nprot.2007.117.