PMID- 17546693
OWN - NLM
STAT- MEDLINE
DCOM- 20080123
LR  - 20131121
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 99
IP  - 1
DP  - 2008 Jan 1
TI  - Acidification of methanol-fed anaerobic granular sludge bioreactors by cobalt 
      deprivation: Induction and microbial community dynamics.
PG  - 49-58
AB  - The acidification of mesophilic (30 degrees C) methanol-fed upflow anaerobic 
      sludge bed (UASB) reactors induced by cobalt deprivation from the influent was 
      investigated by coupling the reactor performance (pH 7.0; organic loading rate 
      4.5 g COD . L(-1) . d(-1)) to the microbial ecology of the bioreactor sludge. The 
      latter was investigated by specific methanogenic activity (SMA) measurements and 
      fluorescence in situ hybridization (FISH) to quantify the abundance of key 
      organisms over time. This study hypothesized that under cobalt limiting 
      conditions, the SMA on methanol of the sludge gradually decreases, which 
      ultimately results in methanol accumulation in the reactor effluent. Once the 
      methanol accumulation surpasses a threshold value (about 8.5 mM for the sludge 
      investigated), reactor acidification occurs because acetogens outcompete 
      methylothrophic methanogens at these elevated methanol concentrations. 
      Methanogens present in granular sludge at the time of the acidification do not 
      use methanol as the direct substrate and are unable to degrade acetate. 
      Methylotrophic/acetoclastic methanogenic activity was found to be lost within 10 
      days of reactor operation, coinciding with the disappearance of the 
      Methanosarcina population. The loss of SMA on methanol can thus be used as an 
      accurate parameter to predict reactor acidification of methanol-fed UASB reactors 
      operating under cobalt limiting conditions.
CI  - (c) 2007 Wiley Periodicals, Inc.
FAU - Fermoso, Fernando G
AU  - Fermoso FG
AD  - Sub-department of Environmental Technology, Wageningen University, 
      "Biotechnion"-Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
FAU - Collins, Gavin
AU  - Collins G
FAU - Bartacek, Jan
AU  - Bartacek J
FAU - O'Flaherty, Vincent
AU  - O'Flaherty V
FAU - Lens, Piet
AU  - Lens P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Sewage)
RN  - 3G0H8C9362 (Cobalt)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Bacteria, Anaerobic/*physiology
MH  - Bioreactors/*microbiology
MH  - Cobalt/*metabolism
MH  - Computer Simulation
MH  - Hydrogen-Ion Concentration
MH  - Methanol/*metabolism
MH  - *Models, Biological
MH  - Sewage/*microbiology
EDAT- 2007/06/05 09:00
MHDA- 2008/01/24 09:00
CRDT- 2007/06/05 09:00
PHST- 2007/06/05 09:00 [pubmed]
PHST- 2008/01/24 09:00 [medline]
PHST- 2007/06/05 09:00 [entrez]
AID - 10.1002/bit.21528 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2008 Jan 1;99(1):49-58. doi: 10.1002/bit.21528.