PMID- 17546997
OWN - NLM
STAT- MEDLINE
DCOM- 20070727
LR  - 20190917
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 55
IP  - 8-9
DP  - 2007
TI  - Experimental and simulation analysis of community structure of nitrifying 
      bacteria in a membrane-aerated biofilm.
PG  - 283-90
AB  - Until now, only few attempts have been made to assess biofilm models simulating 
      microenvironments in a biofilm. As a first step, we compare the microenvironment 
      observed in a membrane aerated biofilm (MAB) to that derived from a 
      two-dimensional computational model with individual ammonia oxidizing bacteria 
      (AOB) and nitrite oxidizing bacteria (NOB) embedded in a continuum EPS matrix. 
      Gradients of oxygen were determined by means of microelectrodes. The change in 
      nitrifying bacterial populations with the biofilm depth was quantified using 
      fluorescence in situ hybridization (FISH) in combination with a confocal laser 
      scanning microscopy (CLSM). Microelectrode measurements revealed that oxic and 
      anoxic or anaerobic regions exist within the MAB. The oxygen profile predicted by 
      the model showed good agreement with that obtained by microelectrode 
      measurements. The oxic part of the biofilm was dominated by NSO190 
      probe-hybridized AOB, which formed relatively large clusters of cells directly on 
      the membrane surface, and by the NOB belonging to genus Nitrobacter sp. On the 
      other hand, NOB belonging to genus Nitrospira sp. were abundant at the 
      oxic-anoxic interface. The model prediction regarding AOB and Nitrobacter sp. 
      distribution was consistent with the experimental counterpart. Measurements of 
      AOB cluster size distribution showed that colonies are slightly larger adjacent 
      to the membrane than at the inner part of the biofilm. The sizes predicted by the 
      current model are larger than those obtained in the experiment, leading to the 
      arguments that some factors not contained in the model would affect the cluster 
      size.
FAU - Matsumoto, S
AU  - Matsumoto S
AD  - Department of Chemical Engineering, Waseda University, Ohkubo 3-4-1, Shinjuku-ku, 
      Tokyo 169-8555, Japan.
FAU - Terada, A
AU  - Terada A
FAU - Aoi, Y
AU  - Aoi Y
FAU - Tsuneda, S
AU  - Tsuneda S
FAU - Alpkvist, E
AU  - Alpkvist E
FAU - Picioreanu, C
AU  - Picioreanu C
FAU - van Loosdrecht, M C M
AU  - van Loosdrecht MC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Biopolymers)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/metabolism
MH  - Bacteria/classification/*growth & development/metabolism
MH  - Biofilms/classification/*growth & development
MH  - Biopolymers/metabolism
MH  - Bioreactors
MH  - Computer Simulation
MH  - Membranes, Artificial
MH  - *Models, Biological
MH  - Nitrites/metabolism
MH  - Oxygen/analysis
EDAT- 2007/06/06 09:00
MHDA- 2007/07/28 09:00
CRDT- 2007/06/06 09:00
PHST- 2007/06/06 09:00 [pubmed]
PHST- 2007/07/28 09:00 [medline]
PHST- 2007/06/06 09:00 [entrez]
AID - 10.2166/wst.2007.269 [doi]
PST - ppublish
SO  - Water Sci Technol. 2007;55(8-9):283-90. doi: 10.2166/wst.2007.269.