PMID- 17548635
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 12
DP  - 2007 Jun 15
TI  - Dendritic cells derived from murine colonic mucosa have unique functional and 
      phenotypic characteristics.
PG  - 7984-93
AB  - Dendritic cells (DCs) residing in different tissues and exposed to different 
      organisms are likely to have different reactivities to their surrounding 
      environment. Many studies use in vitro generated DCs to examine functions of 
      these cells, but such cells may not truly reflect the nature of DCs and their in 
      situ activities in vivo. We have used magnetic label-based technique to isolate 
      colonic DCs to conduct derailed characterization of these cells. Colonic DCs 
      comprise mainly CD11b+ DCs with few CD8alpha+ DCs or plasmacytoid DCs. 
      Functionally, isolated colonic DCs are able to endocytose and process proteins, 
      undergo maturation, and stimulate T cells to proliferate. Importantly, expression 
      of TLRs by colonic DCs is significantly lower than that of their spleen 
      counterparts; however, they appear to be as, or more, responsive to stimulation 
      by oligodeoxynucleotides containing CpG motif based on their cytokine production. 
      We speculate that colonic DCs have unique reactivities differing from DCs 
      residing in other lymphoid tissues and are adapted for the unique 
      microenvironment of the colonic mucosa and that these cells react uniquely to 
      their environment.
FAU - Takenaka, Shunsuke
AU  - Takenaka S
AD  - Center for Gene Therapeutics, Department of Pathology and Molecular Medicine, 
      McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada.
FAU - Safroneeva, Ekaterina
AU  - Safroneeva E
FAU - Xing, Zhou
AU  - Xing Z
FAU - Gauldie, Jack
AU  - Gauldie J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CD11b Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CpG ODN 1826)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - CD11b Antigen/analysis
MH  - CD11c Antigen/analysis
MH  - Colon/*immunology
MH  - DNA/pharmacology
MH  - Dendritic Cells/drug effects/*immunology
MH  - Female
MH  - Gene Expression
MH  - Intestinal Mucosa/*immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Oligodeoxyribonucleotides
MH  - Phenotype
MH  - T-Lymphocytes/*immunology
MH  - Toll-Like Receptors/genetics
EDAT- 2007/06/06 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/06/06 09:00
PHST- 2007/06/06 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/06/06 09:00 [entrez]
AID - 178/12/7984 [pii]
AID - 10.4049/jimmunol.178.12.7984 [doi]
PST - ppublish
SO  - J Immunol. 2007 Jun 15;178(12):7984-93. doi: 10.4049/jimmunol.178.12.7984.