PMID- 17550359
OWN - NLM
STAT- MEDLINE
DCOM- 20070821
LR  - 20070606
IS  - 0931-2668 (Print)
IS  - 0931-2668 (Linking)
VI  - 124
IP  - 3
DP  - 2007 Jun
TI  - The mechanisms determining the nucleolar-organizing regions inactivation of 
      domestic horse chromosomes.
PG  - 163-71
AB  - Cytogenetic investigations of the nucleolar-organizing regions (NORs) show that 
      there is variation in the transcriptional activity of rDNA in many organisms. As 
      a consequence, genetic polymorphism of these regions has been detected. The aim 
      of the present study was to evaluate the hypothetic genetic mechanisms 
      determining the NORs polymorphism of the domestic horse chromosomes. Molecular 
      cytogenetic analyses were carried out on Hucul horses and the following 
      techniques were used: fluorescence in situ hybridization (FISH), telomere primed 
      in situ synthesis (PRINS), in situ nick-translation with HpaII, silver staining 
      (AgNOR) and C-banding technique (CBG). The obtained results suggest that 
      variation in the number and size of silver deposits is related to the number of 
      rDNA copies, DNA methylation and the localization of ribosomal DNA loci in 
      telomeric regions. Moreover, we have found that chromosome pairs 28 and 31 are 
      characterized by higher variation in the NORs number.
FAU - Slota, E
AU  - Slota E
AD  - Department of Immuno- and Cytogenetics, National Research Institute of Animal 
      Production, Balice n. Krakow, Poland. eslota@izoo.krakow.pl
FAU - Wnuk, M
AU  - Wnuk M
FAU - Bugno, M
AU  - Bugno M
FAU - Pienkowska-Schelling, A
AU  - Pienkowska-Schelling A
FAU - Schelling, C
AU  - Schelling C
FAU - Bratus, A
AU  - Bratus A
FAU - Kotylak, Z
AU  - Kotylak Z
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Anim Breed Genet
JT  - Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und 
      Zuchtungsbiologie
JID - 100955807
SB  - IM
MH  - Animals
MH  - Chromosomes, Mammalian/*genetics
MH  - Cytogenetic Analysis/veterinary
MH  - *Gene Expression Regulation
MH  - Horses/*genetics
MH  - Nucleolus Organizer Region/*genetics
EDAT- 2007/06/07 09:00
MHDA- 2007/08/22 09:00
CRDT- 2007/06/07 09:00
PHST- 2007/06/07 09:00 [pubmed]
PHST- 2007/08/22 09:00 [medline]
PHST- 2007/06/07 09:00 [entrez]
AID - JBG642 [pii]
AID - 10.1111/j.1439-0388.2007.00642.x [doi]
PST - ppublish
SO  - J Anim Breed Genet. 2007 Jun;124(3):163-71. doi: 
      10.1111/j.1439-0388.2007.00642.x.