PMID- 17553425
OWN - NLM
STAT- MEDLINE
DCOM- 20070731
LR  - 20191210
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 54
IP  - 5
DP  - 2007 Jun 7
TI  - Activity-regulated N-cadherin endocytosis.
PG  - 771-85
AB  - Enduring forms of synaptic plasticity are thought to require ongoing regulation 
      of adhesion molecules, such as N-cadherin, at synaptic junctions. Little is known 
      about the activity-regulated trafficking of adhesion molecules. Here we 
      demonstrate that surface N-cadherin undergoes a surprisingly high basal rate of 
      internalization. Upon activation of NMDA receptors (NMDAR), the rate of 
      N-cadherin endocytosis is significantly reduced, resulting in an accumulation of 
      N-cadherin in the plasma membrane. Beta-catenin, an N-cadherin binding partner, 
      is a primary regulator of N-cadherin endocytosis. Following NMDAR stimulation, 
      beta-catenin accumulates in spines and exhibits increased binding to N-cadherin. 
      Overexpression of a mutant form of beta-catenin, Y654F, prevents the 
      NMDAR-dependent regulation of N-cadherin internalization, resulting in 
      stabilization of surface N-cadherin molecules. Furthermore, the stabilization of 
      surface N-cadherin blocks NMDAR-dependent synaptic plasticity. These results 
      indicate that NMDAR activity regulates N-cadherin endocytosis, providing a 
      mechanistic link between structural plasticity and persistent changes in synaptic 
      efficacy.
FAU - Tai, Chin-Yin
AU  - Tai CY
AD  - Division of Biology 114-96, California Institute of Technology, Pasadena, CA 
      91125, USA.
FAU - Mysore, Shreesh P
AU  - Mysore SP
FAU - Chiu, Cindy
AU  - Chiu C
FAU - Schuman, Erin M
AU  - Schuman EM
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Cadherins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - COS Cells
MH  - Cadherins/*metabolism
MH  - Cell Membrane/metabolism
MH  - Chlorocebus aethiops
MH  - Dendritic Spines/*metabolism/ultrastructure
MH  - Endocytosis/*physiology
MH  - Hippocampus/cytology/*growth & development/*metabolism
MH  - Humans
MH  - Mice
MH  - Microscopy, Confocal
MH  - Mutation/genetics
MH  - Neuronal Plasticity/physiology
MH  - Organ Culture Techniques
MH  - Patch-Clamp Techniques
MH  - Protein Binding/physiology
MH  - Protein Transport/physiology
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Synapses/*metabolism
MH  - Synaptic Transmission/physiology
MH  - beta Catenin/genetics/metabolism
EDAT- 2007/06/08 09:00
MHDA- 2007/08/01 09:00
CRDT- 2007/06/08 09:00
PHST- 2006/11/02 00:00 [received]
PHST- 2007/03/23 00:00 [revised]
PHST- 2007/05/09 00:00 [accepted]
PHST- 2007/06/08 09:00 [pubmed]
PHST- 2007/08/01 09:00 [medline]
PHST- 2007/06/08 09:00 [entrez]
AID - S0896-6273(07)00347-9 [pii]
AID - 10.1016/j.neuron.2007.05.013 [doi]
PST - ppublish
SO  - Neuron. 2007 Jun 7;54(5):771-85. doi: 10.1016/j.neuron.2007.05.013.