PMID- 17553693
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20181113
IS  - 1044-7431 (Print)
IS  - 1095-9327 (Electronic)
IS  - 1044-7431 (Linking)
VI  - 35
IP  - 3
DP  - 2007 Jul
TI  - Ca2+, CREB and kruppel: a novel KLF7-binding element conserved in mouse and human 
      TRKB promoters is required for CREB-dependent transcription.
PG  - 447-55
AB  - Brain-derived neurotrophic factor (BDNF) signaling through its receptor, trkB, is 
      essential for the proper development and function of the nervous system. Here we 
      identify a novel regulatory element designated TCaRE3 (TRKB Ca(2+) response 
      element 3) required for CREB-dependent TRKB transcription in neurons. 
      TCaRE3-inactivating mutations abolished both Ca(2+)- and cAMP-stimulated TRKB 
      expression, despite the presence of upstream CREs. TCaRE3 mutations also reduced 
      basal expression by at least 80%. Electrophoretic mobility shift assays revealed 
      the presence of a neuronal nuclear factor able to bind TCaRE3 in a 
      sequence-specific manner and we have identified kruppel-like factor 7 (KLF7) as a 
      candidate TCaRE3 transcription factor. Importantly, despite limited overall 
      sequence homology between the promoter regions of the human and mouse TRKB genes, 
      TCaRE3 exhibits 100% sequence identity. Mutation analysis of the human TRKB 
      promoter region demonstrated that the role of TCaRE3 is also conserved, 
      suggesting that the functional interaction between CREB bound to the CREs and 
      KLF7 bound to TCaRE3 is essential for the proper regulation of TRKB in neurons.
FAU - Kingsbury, Tami J
AU  - Kingsbury TJ
AD  - Department of Physiology, University of Maryland School of Medicine, Baltimore, 
      MD 21201, USA.
FAU - Krueger, Bruce K
AU  - Krueger BK
LA  - eng
GR  - R01NS048095/NS/NINDS NIH HHS/United States
GR  - K12 HD043489/HD/NICHD NIH HHS/United States
GR  - K12 HD43489/HD/NICHD NIH HHS/United States
GR  - R01 NS048095-02/NS/NINDS NIH HHS/United States
GR  - T32 NS07375/NS/NINDS NIH HHS/United States
GR  - T32 NS007375/NS/NINDS NIH HHS/United States
GR  - R01 NS048095-03/NS/NINDS NIH HHS/United States
GR  - R01 NS040492/NS/NINDS NIH HHS/United States
GR  - R01 NS048095-01A1/NS/NINDS NIH HHS/United States
GR  - R01 NS048095/NS/NINDS NIH HHS/United States
GR  - R01NS40492/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070504
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Klf7 protein, mouse)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Chromatin Immunoprecipitation/methods
MH  - Cyclic AMP Response Element-Binding Protein/*physiology
MH  - Embryo, Mammalian
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Kruppel-Like Transcription Factors/*metabolism
MH  - Mice
MH  - Mutation/physiology
MH  - Neurons/metabolism
MH  - Promoter Regions, Genetic/physiology
MH  - Protein Binding/physiology
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, trkB/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Signal Transduction
MH  - Transcription, Genetic
MH  - Transfection/methods
PMC - PMC2042965
MID - NIHMS27294
EDAT- 2007/06/08 09:00
MHDA- 2007/09/27 09:00
PMCR- 2008/07/01
CRDT- 2007/06/08 09:00
PHST- 2006/12/10 00:00 [received]
PHST- 2007/03/17 00:00 [revised]
PHST- 2007/04/17 00:00 [accepted]
PHST- 2007/06/08 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/06/08 09:00 [entrez]
PHST- 2008/07/01 00:00 [pmc-release]
AID - S1044-7431(07)00100-5 [pii]
AID - 10.1016/j.mcn.2007.04.004 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2007 Jul;35(3):447-55. doi: 10.1016/j.mcn.2007.04.004. Epub 
      2007 May 4.