PMID- 17557153
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20231105
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 390
IP  - 3
DP  - 2008 Feb
TI  - Growth cone response to ephrin gradients produced by microfluidic networks.
PG  - 809-16
AB  - A microfluidic network (microFN) etched into a silicon wafer was used to deliver 
      protein solutions containing different concentrations of the axonal guidance 
      molecule ephrinA5 onto a silicone stamp. In a subsequent microcontact printing 
      (microCP) step, the protein was transferred onto a polystyrene culture dish. In 
      this way, stepwise substrate-bound concentration gradients of ephrinA5 were 
      fabricated spanning a total distance of 320 microm. We tested the response of 
      chick retinal ganglion cell (RGC) axons, which are guided in vivo by ephrin 
      gradients, to these in vitro gradients. Temporal, but not nasal axons stop at a 
      distinct zone in the gradient, which is covered with a certain surface density of 
      substrate-bound ephrinA5. Within the temporal RGC population, all axons respond 
      uniformly to the gradients tested. The position of the stop zone depends on the 
      slope of the gradient with axons growing further into the gradient in shallow 
      gradients than in steep gradients. However, axons stop at lower ephrinA5 
      concentrations in shallow gradients than in steep gradients, indicating that the 
      growth cone can adjust its sensitivity during the detection of a concentration 
      gradient of ephrinA5.
FAU - Lang, Susanne
AU  - Lang S
AD  - Max-Planck-Institut fur Entwicklungsbiologie, Spemannstrasse 35, 72076, Tubingen, 
      Germany.
FAU - von Philipsborn, Anne C
AU  - von Philipsborn AC
FAU - Bernard, Andre
AU  - Bernard A
FAU - Bonhoeffer, Friedrich
AU  - Bonhoeffer F
FAU - Bastmeyer, Martin
AU  - Bastmeyer M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070608
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Ephrin-A5)
RN  - 0 (Ephrins)
RN  - 0 (Polystyrenes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Cell Culture Techniques/methods
MH  - Chickens
MH  - Dimethylpolysiloxanes/chemistry
MH  - Ephrin-A5/chemistry
MH  - Ephrins/*metabolism
MH  - Equipment Design
MH  - Growth Cones/*physiology
MH  - *Microfluidic Analytical Techniques
MH  - *Microfluidics
MH  - Polystyrenes/chemistry
MH  - Retinal Ganglion Cells/cytology
MH  - Silicones/chemistry
MH  - Time Factors
PMC - PMC2755754
EDAT- 2007/06/09 09:00
MHDA- 2008/05/23 09:00
PMCR- 2009/10/02
CRDT- 2007/06/09 09:00
PHST- 2007/04/03 00:00 [received]
PHST- 2007/05/10 00:00 [accepted]
PHST- 2007/05/03 00:00 [revised]
PHST- 2007/06/09 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2007/06/09 09:00 [entrez]
PHST- 2009/10/02 00:00 [pmc-release]
AID - 1363 [pii]
AID - 10.1007/s00216-007-1363-3 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2008 Feb;390(3):809-16. doi: 10.1007/s00216-007-1363-3. Epub 
      2007 Jun 8.