PMID- 17557250
OWN - NLM
STAT- MEDLINE
DCOM- 20090323
LR  - 20070608
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 3
DP  - 2007 Jun
TI  - [Influence of interphase nuclei preparation techniques on fluorescence in situ 
      hybridization results in solid tumors].
PG  - 331-3
AB  - OBJECTIVE: To establish a technology platform for the preparation of interphase 
      nuclei for the fluorescence in situ hybridization (FISH) detection of solid tumor 
      tissues. METHODS: The centromere probe of chromosome 3 was labeled by the random 
      primer technique, and then hybridized to interphase nuclei prepared by six 
      different methods in order to study the influence on FISH detection. RESULTS: 
      Each method of slide preparation had its own characteristic, and could be used 
      according to different needs. As regards to FISH, collagenase method got the best 
      results. Whereas for frozen samples or small tissues, to prepare printing slides 
      was more applicable. CONCLUSION: The comparison of different slide preparation 
      methods lays a technology foundation for the FISH application in cancer 
      researches and clinical diagnosis of solid tumors.
FAU - Han, Ya-ling
AU  - Han YL
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100021 PR 
      China.
FAU - Xu, Xin
AU  - Xu X
FAU - Cai, Yan
AU  - Cai Y
FAU - Wang, Ming-rong
AU  - Wang MR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - Animals
MH  - Cell Fractionation/*methods
MH  - *Cell Nucleus/metabolism
MH  - Collagenases/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Interphase
MH  - Neoplasms/*genetics/*pathology
EDAT- 2007/06/09 09:00
MHDA- 2009/03/24 09:00
CRDT- 2007/06/09 09:00
PHST- 2007/06/09 09:00 [pubmed]
PHST- 2009/03/24 09:00 [medline]
PHST- 2007/06/09 09:00 [entrez]
AID - 940624075 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Jun;24(3):331-3.