PMID- 17557338
OWN - NLM
STAT- MEDLINE
DCOM- 20071129
LR  - 20181201
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 25
IP  - 11
DP  - 2007 Nov
TI  - In vitro and in vivo optimization of impaction allografting by demineralization 
      and addition of rh-OP-1.
PG  - 1425-37
AB  - Impaction allografting is a bone tissue engineering technique currently used in 
      lower limb reconstruction orthopedic surgery. Our hypothesis was that biological 
      optimization can be achieved by demineralization and addition of osteogenic 
      protein-1 (OP-1) to the allograft. The objective of our in vitro study was to 
      evaluate human mesenchymal stem cell (MSC) proliferation (Alamar Blue assay, 
      titrated thymidine assay, total DNA Hoechst 33258, and scanning electron 
      microscopy) and osteogenic differentiation (alkaline phosphatase assay) in two 
      types of impacted carrier, namely, demineralized bone matrix (DBM) and insoluble 
      collagenous bone matrix (ICBM), with or without OP-1. The objective in vivo was 
      to compare the osteogenic potential of impacted DBM with or without OP-1, with 
      that of impacted fresh frozen allograft (FFA), again with or without OP-1. DBM + 
      OP-1 optimized osteoinduction and significantly improved (p < 0.05) proliferation 
      and differentiation in comparison to the majority of all other graft preparation 
      in vitro. In addition, DBM + OP-1 was significantly superior, with regard to 
      osteogenesis, compared to the impacted FFA alone (p < 0.001), FFA + OP-1 (p = 
      0.01) and DBM alone (p = 0.02) in vivo. We propose that partial demineralization 
      and addition of OP-1 provides a good method for improving the osteoinductive 
      properties of fresh allograft currently used in the impaction grafting technique.
CI  - (c) 2007 Orthopaedic Research Society.
FAU - Tsiridis, Eleftherios
AU  - Tsiridis E
AD  - Biomaterials and Biomimetics Group, King's College London, University of London, 
      Guy's Hospital, London SE1 9RT, United Kingdom. etsiridis@doctors.org.uk
FAU - Ali, Zubier
AU  - Ali Z
FAU - Bhalla, Amit
AU  - Bhalla A
FAU - Heliotis, Manolis
AU  - Heliotis M
FAU - Gurav, Neelam
AU  - Gurav N
FAU - Deb, Sanjukta
AU  - Deb S
FAU - DiSilvio, Lucy
AU  - DiSilvio L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (BMP7 protein, human)
RN  - 0 (Bmp7 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - *Bone Demineralization Technique
MH  - Bone Marrow Cells/drug effects/ultrastructure
MH  - Bone Matrix/*transplantation
MH  - Bone Morphogenetic Protein 7
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Bone Transplantation/instrumentation/*methods
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/drug effects/enzymology/ultrastructure
MH  - Osteogenesis/drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering/instrumentation/*methods
MH  - Transplantation, Homologous
EDAT- 2007/06/09 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/06/09 09:00
PHST- 2007/06/09 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/06/09 09:00 [entrez]
AID - 10.1002/jor.20387 [doi]
PST - ppublish
SO  - J Orthop Res. 2007 Nov;25(11):1425-37. doi: 10.1002/jor.20387.