PMID- 17558540 OWN - NLM STAT- MEDLINE DCOM- 20071128 LR - 20070827 IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 152 IP - 9 DP - 2007 TI - Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiaceae. PG - 1603-11 AB - A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chenopodium quinoa Willd.), Solanaceae (Nicotiana benthamiana Domin, and N. clevlandii Gray.), and Apiaceae (Anethum graveolens L., Apium graveolens L. var. dulce (Miller)), Daucus carota L. subspecies sativus (Hoffm.) Arcang., and Petroselinum crispum (Miller) Nyman ex A. W. Hilland). The virus contained flexuous rods with an ssRNA genome approximately 9.5-10 k nts and a CP ( approximately 35 kDa) that reacted to a universal potyvirus monoclonal antibody in Western blot analysis. The sequenced genomic 3'-end ( approximately 1850 nt) contained a potyvirus genomic arrangement that included the 3'-terminus of the NIb (nuclear inclusion) gene, the CP (coat protein) gene, and a 3'-UTR (untranslated region) attached to a poly(A)tail. The CP amino acids had between 54 and 70% identity with 12 selected members from the genus Potyvirus. Phylogenetically, the Alaskan potyvirus clustered with three other apiaceous potyviruses from Australia. The novel Alaskan virus was confined to A. lucida L. and A. genuflexa Nutt. in nature, and was classified in the genus Potyvirus, family Potyviridae, and in part named after its natural plant hosts, angelica virus Y (AnVY). FAU - Robertson, N L AU - Robertson NL AD - USDA, ARS, Subarctic Agricultural Research Service, Arctic Plant Germplasm Research and Introduction, Palmer, AK 99645, USA. pfnlr@uaa.alaska.edu LA - eng SI - GENBANK/EF488740 SI - GENBANK/EF488741 PT - Journal Article DEP - 20070611 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 SB - IM MH - Angelica/*virology MH - Molecular Sequence Data MH - Phylogeny MH - Plant Diseases/virology MH - Potyvirus/*classification/genetics/isolation & purification EDAT- 2007/06/15 09:00 MHDA- 2007/12/06 09:00 CRDT- 2007/06/15 09:00 PHST- 2006/12/21 00:00 [received] PHST- 2007/05/04 00:00 [accepted] PHST- 2007/06/15 09:00 [pubmed] PHST- 2007/12/06 09:00 [medline] PHST- 2007/06/15 09:00 [entrez] AID - 10.1007/s00705-007-1003-4 [doi] PST - ppublish SO - Arch Virol. 2007;152(9):1603-11. doi: 10.1007/s00705-007-1003-4. Epub 2007 Jun 11.