PMID- 17558715
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20070611
IS  - 0882-0139 (Print)
IS  - 0882-0139 (Linking)
VI  - 36
IP  - 3
DP  - 2007
TI  - Neutrophils induce the maturation of immature dendritic cells: a regulatory role 
      of neutrophils in adaptive immune responses.
PG  - 337-50
AB  - Th1-type immune cytokines are essential to establish adaptive immunity against 
      various microbial pathogens, including Escherichia coli, which cause most urinary 
      tract infections (UTIs). Dendritic cells (DCs) are vital to initiate Th1 
      immunity, while neutrophils, also referred to here as polymorphonuclear 
      leukocytes (PMN) are reported to be involved in Th1 immunity initiation by 
      secreting several chemokines and cytokines. We found that lipopolysaccharide 
      (LPS)-triggered PMN (LPS-PMN) in vitro induced strong up-regulation of DCs 
      surface markers CD40, CD80, MHC-II (Iab), and CD86 either by secreting soluble 
      factors, such as TNF-alpha, or by PMN-DC cellular contact. LPS-PMN also 
      stimulated DCs to produce IL-12 and TNF-alpha. Furthermore, purified DCs 
      activated by LPS-PMN were able to present specific antigen to T cells and drive 
      Th1 differentiation by producing large amount of IFN-gamma but low amount of 
      IL-4. Our results suggest a regulatory role of PMN for DCs function in adaptive 
      immune responses, thereby providing a link between innate and adaptive immunity.
FAU - Xiaoxiao, Wan
AU  - Xiaoxiao W
AD  - Department of Urology, Jinshan Hospital, Fudan University, Shanghai, China.
FAU - Sibiao, Yue
AU  - Sibiao Y
FAU - Xiaopeng, Xiong
AU  - Xiaopeng X
FAU - Ping, Zheng
AU  - Ping Z
FAU - Gang, Chen
AU  - Gang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis/genetics
MH  - Dendritic Cells/drug effects/*immunology/*physiology
MH  - Gene Expression Regulation/*drug effects
MH  - Immunity, Cellular/*immunology
MH  - Lipopolysaccharides/antagonists & inhibitors
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Neutrophils/immunology/*physiology
EDAT- 2007/06/15 09:00
MHDA- 2007/09/27 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - 779284254 [pii]
AID - 10.1080/08820130601109719 [doi]
PST - ppublish
SO  - Immunol Invest. 2007;36(3):337-50. doi: 10.1080/08820130601109719.