PMID- 17559879
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20081121
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 142
IP  - 1
DP  - 2007 Sep
TI  - The mechanism of how anti-IL-18 prevents concanavalin-A-induced hepatic fibrosis 
      on a mouse model.
PG  - 175-83
AB  - BACKGROUND: The administration of concanavalin A (ConA) induces severe hepatic 
      fibrosis in mice. Tumor necrosis factor-alpha (TNF-alpha), interferon-gamma 
      (IFN-gamma) and interleukin 4 (IL-4) were the key cytokines involved in the 
      process. The aim of this research was to explore the effects and the mechanisms 
      of IL-18 and anti-IL-18 on hepatic fibrosis in a ConA induced hepatic fibrosis 
      model in BABL-C mice. MATERIALS AND METHODS: One hundred eighty BABL-C mice were 
      randomly divided into five groups (Group a, b, c, d, e). The mice were 
      administered saline, immunoglobulin G, ConA, IL-18 + ConA, Anti-IL-18 + ConA, 
      respectively. At 1, 7, 14, 21 wk, the levels of serum alanine aminotransferase, 
      TNF-alpha, IFN-gamma, IL-4, matrix metalloproteinase (MMP)-2-RNA, and tissue 
      inhibitor of metalloproteinase-1-mRNA were measured. RESULTS: The levels of serum 
      TNF-alpha and IFN-gamma detected in the IL-18 + ConA group was higher than in the 
      anti-IL-18 + ConA group (P < 0.05). Similarly, the levels of MMP-2-RNA and tissue 
      inhibitor of metalloproteinase-1-mRNA expressed in IL-18 + ConA group was higher 
      than in the anti-IL-18 + ConA group (P < 0.05). A majority of these cytokines was 
      secreted by CD4(+)T cells. CONCLUSIONS: The immunological response to hepatic 
      fibrosis by repeated injection of ConA in the mouse model was aggravated by IL-18 
      and blocked by anti-IL-18.
FAU - Zhang, Yewei
AU  - Zhang Y
AD  - The Liver Transplantation Center of the First Affiliated Hospital, Nanjing 
      Medical University, Nanjing, China.
FAU - Li, Ping
AU  - Li P
FAU - Li, Guoqiang
AU  - Li G
FAU - Huang, Xinli
AU  - Huang X
FAU - Meng, Qingyang
AU  - Meng Q
FAU - Lau, Wan Y
AU  - Lau WY
FAU - Wang, Xuehao
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070607
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-18)
RN  - 0 (Mitogens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11028-71-0 (Concanavalin A)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology/*pharmacology
MH  - Apoptosis/drug effects
MH  - CD4-Positive T-Lymphocytes/drug effects/metabolism/pathology
MH  - Cell Proliferation/drug effects
MH  - Concanavalin A
MH  - Disease Models, Animal
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-18/*immunology/*pharmacology
MH  - Interleukin-4/genetics/metabolism
MH  - Liver/drug effects/metabolism/pathology
MH  - Liver Cirrhosis/chemically induced/pathology/*prevention & control
MH  - Male
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitogens
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
EDAT- 2007/06/15 09:00
MHDA- 2007/10/03 09:00
CRDT- 2007/06/15 09:00
PHST- 2006/10/22 00:00 [received]
PHST- 2007/01/16 00:00 [revised]
PHST- 2007/01/23 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0022-4804(07)00033-9 [pii]
AID - 10.1016/j.jss.2007.01.024 [doi]
PST - ppublish
SO  - J Surg Res. 2007 Sep;142(1):175-83. doi: 10.1016/j.jss.2007.01.024. Epub 2007 Jun 
      7.