PMID- 17560028
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20181113
IS  - 0304-3940 (Print)
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 421
IP  - 2
DP  - 2007 Jun 27
TI  - Differential regulation of glutamate receptors in trigeminal ganglia following 
      masseter inflammation.
PG  - 91-5
AB  - The present study examined whether N-methyl-D-aspartate receptor (NMDAR), 
      5-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) 
      subunits and group I metabotropic glutamate receptors (mGluRs) are constitutively 
      expressed in trigeminal ganglia (TG) using Western blot analysis in male 
      Sprague-Dawley rats. We then investigated whether experimental induction of 
      masseter inflammation influences glutamate receptor expressions by comparing the 
      protein levels from naive rats to those from complete Freund's adjuvant (CFA) 
      inflamed rats. Our results showed that NMDAR1 (NR1), NMDAR2A (NR2A), NMDAR2B 
      (NR2B), AMPAR1 (GluR1) and AMPAR2 (GluR2) subunits, and group I metabotropic 
      glutamate receptor, mGluR5, are constitutively expressed in TG. Masseter 
      inflammation significantly down-regulated NR1 subunit expression that persisted 
      to 7 days post-CFA inflammation. NR2A and NR2B expressions were not significantly 
      changed. GluR1 receptor subunit expression was slightly increased in TG 3 days 
      after CFA-induced inflammation, but the change was not statistically significant. 
      GluR2 protein level was not affected by CFA inflammation. The level of mGluR5 
      protein was significantly up-regulated in TG 3 days after CFA-induced masseter 
      inflammation. There were no inflammation-induced changes in any of the proteins 
      we analyzed in the contralateral, non-inflamed TG. These results suggested that 
      muscle inflammation differentially modulates glutamate receptor subunits at the 
      primary afferent level in male rats and that these inflammation-induced 
      transcriptional changes may contribute to functionally different aspects of 
      craniofacial muscle pain.
FAU - Lee, Jongseok
AU  - Lee J
AD  - Department of Biomedical Sciences, Program in Neuroscience, University of 
      Maryland Baltimore, School of Dentistry, 650 W. Baltimore Street, Baltimore, 
      Maryland 21201, USA.
FAU - Ro, Jin Y
AU  - Ro JY
LA  - eng
GR  - R01 DE016062/DE/NIDCR NIH HHS/United States
GR  - R01 DE016062-03/DE/NIDCR NIH HHS/United States
GR  - D16062/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070525
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Receptors, Glutamate)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Animals
MH  - Blotting, Western/methods
MH  - Freund's Adjuvant
MH  - Gene Expression Regulation/*physiology
MH  - Male
MH  - *Masseter Muscle
MH  - Myositis/chemically induced/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Glutamate/*metabolism
MH  - Time Factors
MH  - Trigeminal Ganglion/*metabolism
PMC - PMC2956190
MID - NIHMS243634
EDAT- 2007/06/15 09:00
MHDA- 2007/09/29 09:00
PMCR- 2010/10/18
CRDT- 2007/06/15 09:00
PHST- 2007/04/12 00:00 [received]
PHST- 2007/05/17 00:00 [revised]
PHST- 2007/05/18 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
PHST- 2010/10/18 00:00 [pmc-release]
AID - S0304-3940(07)00613-1 [pii]
AID - 10.1016/j.neulet.2007.05.031 [doi]
PST - ppublish
SO  - Neurosci Lett. 2007 Jun 27;421(2):91-5. doi: 10.1016/j.neulet.2007.05.031. Epub 
      2007 May 25.