PMID- 17560530
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20111117
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 34
IP  - 5
DP  - 2007 May
TI  - The distribution of repetitive DNAs along chromosomes in plants revealed by 
      self-genomic in situ hybridization.
PG  - 437-48
AB  - The distribution of repetitive DNAs along chromosomes is one of the crucial 
      elements for understanding the organization and the evolution of plant genomes. 
      Using a modified genomic in situ hybridization (GISH) procedure, fluorescence in 
      situ hybridization (FISH) with genomic DNA to their own chromosomes (called 
      self-genomic in situ hybridization, self-GISH) was carried out in six selected 
      plant species with different genome size and amount of repetitive DNA. Nonuniform 
      distribution of the fluorescent labeled probe DNA was observed on the chromosomes 
      of all the species that were tested. The signal patterns varied among species and 
      were related to the genome size. The chromosomes of the small Arabidopsis genome 
      were labeled almost only in the pericentromeric regions and the nucleolus 
      organizer regions (NORs). The signals in the relatively small genomes, rice, 
      sorghum, and Brassica oleracea var. capitata L., were dispersed along the 
      chromosome lengths, with a predominant distribution in the pericentromeric or 
      proximal regions and some heterochromatic arms. All chromosomes of the large 
      genomes, maize and barley, were densely labeled with strongly labeled regions and 
      weakly labeled or unlabeled regions being arranged alternatively throughout the 
      lengths. In addition, enhanced signal bands were shown in all pericentromeres and 
      the NORs in B. oleracea var. capitata and in all pericentromeric regions and 
      certain intercalary sites in barley. The enhanced signal band pattern in barley 
      was found consistent with the N-banding pattern of this species. The GISH with 
      self-genomic DNA was compared with FISH with C(o)t-1 DNA in rice, and their 
      signal patterns are found to be basically consistent. Our results showed that the 
      self-GISH signals actually reflected the hybridization of genomic repetitive DNAs 
      to the chromosomes, thus the self-GISH technique would be useful for revealing 
      the distribution of the regions where repetitive DNAs concentrate along 
      chromosomes and some chromatin differentiation associated with repetitive DNAs in 
      plants.
FAU - She, Chaowen
AU  - She C
AD  - Department of Biology, Huaihua University, Huaihua 418008, China. 
      shechaowen@tom.com
FAU - Liu, Jingyu
AU  - Liu J
FAU - Diao, Ying
AU  - Diao Y
FAU - Hu, Zhongli
AU  - Hu Z
FAU - Song, Yunchun
AU  - Song Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
RN  - 0 (Chromatin)
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromatin/genetics
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/*genetics
MH  - *Genomics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Plant Cells
MH  - Plants/*genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 2007/06/15 09:00
MHDA- 2009/04/28 09:00
CRDT- 2007/06/15 09:00
PHST- 2006/06/05 00:00 [received]
PHST- 2006/07/09 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S1673-8527(07)60048-4 [pii]
AID - 10.1016/S1673-8527(07)60048-4 [doi]
PST - ppublish
SO  - J Genet Genomics. 2007 May;34(5):437-48. doi: 10.1016/S1673-8527(07)60048-4.