PMID- 17560570
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20070903
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 83
IP  - 2
DP  - 2007 Oct
TI  - Dynamic changes in conjunctival dendritic cell numbers, anatomical position and 
      phenotype during experimental allergic conjunctivitis.
PG  - 216-23
AB  - Dendritic cells (DCs) are a subset of antigen-presenting cells (APCs) that are 
      involved in the initiation and control of the immune response to antigens present 
      at the interface with the environment. A limited number of groups have studied 
      DCs in human and animal conjunctiva but no data is available concerning the 
      different DC subsets present in the conjunctival tissue. The aims of this study 
      are to characterize the phenotypes and numbers of DCs present in the murine model 
      of allergic conjunctivitis using the technique of immunohistochemistry so as to 
      aid the understanding of the mechanisms involved in allergic eye disease. A 
      double immunofluorescence method was used to analyze the phenotypic distribution 
      and density of DC subsets in the mouse conjunctival tissues of the allergic model 
      using a panel of antibodies: CD11c, as a general marker of DCs, coupled with 
      another DC subset marker such as Langerin for Langerhans cells (LCs), CD11b for 
      myeloid DCs (mDCs) and mPDCA-1 for plasmacytoid DCs (pDCs). In the naive 
      conjunctiva, mDCs were consistently detected in the subepithelial layer and 
      substantia propria. In the epithelium and the subepithelial layer, very few LCs 
      and virtually no pDCs were observed. Following allergen challenge, there was a 
      marked influx of mDCs and pDCs, but no LCs, into the subepithelial layer and 
      throughout the substantia propria. These results indicate that conjunctival DC 
      subsets may play an important role in the immune-regulatory processes involved in 
      the inflammatory component of allergic conjunctivitis.
FAU - Ohbayashi, Masaharu
AU  - Ohbayashi M
AD  - Department of Ophthalmology and Medicine, Emory University School of Medicine & 
      Emory Eye Center, Atlanta, GA 30322, USA.
FAU - Manzouri, Bita
AU  - Manzouri B
FAU - Flynn, Tom
AU  - Flynn T
FAU - Toda, Masako
AU  - Toda M
FAU - Ikeda, Yoshifumi
AU  - Ikeda Y
FAU - Nakamura, Takao
AU  - Nakamura T
FAU - Ono, Santa Jeremy
AU  - Ono SJ
LA  - eng
PT  - Journal Article
DEP - 20070510
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/immunology/pathology
MH  - Conjunctiva/*immunology/pathology
MH  - Conjunctivitis, Allergic/*immunology/pathology
MH  - Dendritic Cells/*immunology/pathology
MH  - Disease Models, Animal
MH  - Eosinophils/immunology/pathology
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Mice
MH  - Mice, Inbred A
MH  - Microscopy, Fluorescence
EDAT- 2007/06/15 09:00
MHDA- 2007/10/25 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/04/20 00:00 [received]
PHST- 2007/04/23 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0014-4800(07)00069-X [pii]
AID - 10.1016/j.yexmp.2007.04.007 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2007 Oct;83(2):216-23. doi: 10.1016/j.yexmp.2007.04.007. Epub 
      2007 May 10.