PMID- 17560940
OWN - NLM
STAT- MEDLINE
DCOM- 20070807
LR  - 20070618
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 359
IP  - 3
DP  - 2007 Aug 3
TI  - Regulation of c-myc through intranuclear localization of its RNA subspecies.
PG  - 806-10
AB  - We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies 
      in human COLO 320DM tumor cells. Although the FISH procedure removed the majority 
      of RNAs from the nucleolus, c-myc RNA continued to be detected in both the 
      nucleoplasm and nucleolus. This finding suggests stable association between c-myc 
      RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be 
      temporally regulated by cell-cycle progression. Hybridization with exon- and 
      strand-specific RNA probes indicated that the non-protein coding exon 1 plays a 
      novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA 
      targeting exon 2 localized only with nucleoplasmic foci, where it might interact 
      with the sense strand. Thus, c-myc gene expression may be regulated by 
      intranuclear localization of its RNA.
FAU - Shimizu, Noriaki
AU  - Shimizu N
AD  - Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, 
      Higashi-Hiroshima 739-8521, Japan. shimizu@hiroshima-u.ac.jp
FAU - Kawamoto, June-ko
AU  - Kawamoto JK
FAU - Utani, Koh-ichi
AU  - Utani K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070604
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Nucleus/*genetics/metabolism
MH  - Exons/genetics
MH  - Gene Dosage/*genetics
MH  - Gene Expression Regulation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Proto-Oncogene Proteins c-myc/*genetics/*metabolism
MH  - RNA/genetics/*metabolism
MH  - *RNA Transport
EDAT- 2007/06/15 09:00
MHDA- 2007/08/08 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/05/16 00:00 [received]
PHST- 2007/05/29 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/08/08 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0006-291X(07)01185-0 [pii]
AID - 10.1016/j.bbrc.2007.05.201 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Aug 3;359(3):806-10. doi: 
      10.1016/j.bbrc.2007.05.201. Epub 2007 Jun 4.