PMID- 17561027
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20090115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1157
IP  - 1-2
DP  - 2007 Jul 20
TI  - Capillary high-performance liquid chromatography with mass spectrometry for 
      simultaneous determination of major flavonoids, iridoid glucosides and saponins 
      in Flos Lonicerae.
PG  - 217-26
AB  - Flos Lonicerae, a traditional herbal medicine, has been used in China to treat 
      some inflammatory disease. Several different classes of compounds have been 
      separated from the herb to assess their pharmacological activities. Among these 
      classes, flavonoids, iridoid glycosides and saponins have been well studied and 
      may be responsible for its clinical application. Therefore, quality control of 
      Flos Lonicerae is an important issue for drug safety and validity evaluations. A 
      quantitative method consisting of solid phase extraction followed by capillary 
      high-performance liquid chromatography-diode array detection/electrospray 
      ionization mass spectrometry (capillary HPLC-DAD/ESI-MS) was developed for 
      simultaneously assay of 24 compounds in Flos Lonicerae. Under optimized capillary 
      HPLC-DAD/ESI-MS conditions, these compounds, including nine flavonoids, eight 
      iridoid glucosides and seven saponins, were separated with high efficiency in the 
      selected-ion monitoring (SIM) mode. Linearity of the method was good with 
      correlation coefficients (r(2)) in the range of 0.9935-0.9998 and detection 
      limits were lower than 2.57 ng/mL for most of analytes. The obtained recoveries 
      varied between 91.0 and 108.7% with the relative standard deviations (RSDs) 
      within 8.74% (n=3). The capillary HPLC-DAD/ESI-MS method was also successfully 
      applied to the analysis of these compounds in five species of Flos Lonicerae. It 
      was demonstrated to be a powerful tool for comprehensive analysis of herbal 
      medicines, owing to its exclusive selectivity and excellent sensitivity.
FAU - Chen, Jun
AU  - Chen J
AD  - Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, 
      Ministry of Education, No. 24 Tongjia Lane, Nanjing 210009, China.
FAU - Song, Yue
AU  - Song Y
FAU - Li, Ping
AU  - Li P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070526
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Glucosides)
RN  - 0 (Saponins)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Flavonoids/*analysis
MH  - Glucosides/*analysis
MH  - Reproducibility of Results
MH  - Saponins/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2007/06/15 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/01/25 00:00 [received]
PHST- 2007/04/28 00:00 [revised]
PHST- 2007/05/02 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0021-9673(07)00857-6 [pii]
AID - 10.1016/j.chroma.2007.05.063 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Jul 20;1157(1-2):217-26. doi: 10.1016/j.chroma.2007.05.063. 
      Epub 2007 May 26.