PMID- 17563405
OWN - NLM
STAT- MEDLINE
DCOM- 20080904
LR  - 20070612
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 6
IP  - 2
DP  - 2007 Jun
TI  - High production of IL-18 by dendritic cells induced by sera from patients with 
      primary antibody deficiency.
PG  - 59-65
AB  - Predominantly antibody deficiencies are a category of primary immunodeficiency 
      diseases, which consist of several rare disorders such as common variable 
      immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA). We evaluated the 
      effects of CVID and XLA patients' sera as a source of microenviromental factors 
      on maturation and function of monocyte-derived DCs. Blood was collected from 10 
      CVID and 5 XLA patients before immunoglobulin replacement therapy and also from 8 
      healthy volunteers in order to obtain necessary sera for this study. Monocyte 
      derived DCs were generated from blood cells obtained from healthy volunteers in 
      the presence of GM-CSF, IL-4 and 10% serum concentrations from cases and 
      controls. Immature DCs were incubated with monocyte conditioned medium (MCM) and 
      TNF- in order to generate mature DCs. Interleukin 18 (IL-18) production by 
      CD40L-activated mature DCs was measured after 24 hours of culture in vitro.IL-18 
      production by DCs generated in the presence of CVID and XLA patients' sera were 
      6.75+/-2.59 and 7.08+/-1.75 ng/ml, respectively, which were significantly higher 
      than normal serum conditioned DCs (3.55+/-0.68) ng/ml. These results suggest that 
      the sera of patients with predominantly antibody deficiencies may contain soluble 
      factor(s) that can induce a significant increase in IL-18 production by DCs.
FAU - Nourizadeh, Maryam
AU  - Nourizadeh M
AD  - Immunology, Asthma and Allergy Research Institute, Medical Sciences/Tehran 
      University, Tehran, Iran.
FAU - Aghamohammadi, Asghar
AU  - Aghamohammadi A
FAU - Moazzeni, Seyed Mohammad
AU  - Moazzeni SM
FAU - Mahdavi, Mahdi
AU  - Mahdavi M
FAU - Rezaei, Nima
AU  - Rezaei N
FAU - Hadjati, Jamshid
AU  - Hadjati J
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Agammaglobulinemia/blood/immunology/physiopathology
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Common Variable Immunodeficiency/*blood/immunology/physiopathology
MH  - Culture Media, Conditioned
MH  - Dendritic Cells/immunology/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulins/blood/deficiency
MH  - Interleukin-18/agonists/*biosynthesis/immunology
MH  - Monocytes/cytology/immunology/metabolism
MH  - Serum/immunology
MH  - Th1 Cells/immunology/metabolism
EDAT- 2007/06/15 09:00
MHDA- 2008/09/05 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2008/09/05 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - 06025969 [pii]
PST - ppublish
SO  - Iran J Allergy Asthma Immunol. 2007 Jun;6(2):59-65.