PMID- 17566625
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20220129
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 237
IP  - 1-3
DP  - 2007 Jul 31
TI  - Styrene-oxide N-terminal valine haemoglobin adducts in reinforced plastic 
      workers: possible influence of genetic polymorphism of drug-metabolising enzymes.
PG  - 58-64
LID - S0300-483X(07)00260-0 [pii]
LID - 10.1016/j.tox.2007.04.019 [doi]
AB  - Styrene is one of the most important organic chemicals used worldwide. In humans, 
      styrene metabolism involves oxidation by cytochrome P450 monooxygenases (CYPs) to 
      styrene-7,8-oxide, an epoxide thought to be responsible for the genotoxic effects 
      of styrene exposure, and detoxification by means of epoxide hydrolase (mEH) and 
      glutathione S-transferases (GSTs). The objective of this study was to investigate 
      if genetic polymorphisms of metabolic enzymes modulate the level of urinary 
      styrene metabolites and styrene oxide adducts with N-terminal valine of human 
      globin (SO-Hb) in 75 workers occupationally exposed to styrene and 77 unexposed 
      controls. The mean air concentration of styrene in the breathing zone of workers 
      (30.4ppm) was higher than the threshold limit value of 20ppm recommended by the 
      American Conference of Governmental Industrial Hygienists (ACGIH), and the 
      biological exposure index adopted by the ACGIH for exposure to styrene prior to 
      the next shift (MA+PGA=400mg/g creatinine) was exceeded, indicating that styrene 
      exposure for this group of workers was higher than recommended. A highly 
      significant correlation was observed between styrene concentration in the 
      breathing zone and the MA+PGA in urine of workers (r=0.85, P<0.001). The levels 
      of SO-Hb adducts in exposed workers were significantly increased as compared with 
      controls, although no difference was observed between subjects stratified as high 
      and medium exposure categories based on MA+PGA excretion. Regarding the effect of 
      the genetic polymorphisms we found that the level of SO-Hb adducts might be 
      modulated by the predicted mEH enzymatic activity in the exposed workers. From 
      our data we conclude that SO-Hb adduct measurement is a complementary method to 
      MA+PG measurement for assessing exposure to styrene at occupational and 
      environmental levels, which reflects a more extensive exposure period.
FAU - Teixeira, J P
AU  - Teixeira JP
AD  - National Institute of Health, Centre of Occupational and Environmental Health, 
      Praca Coronel Pacheco, 15, 4050-453 Porto, Portugal. Electronic address: 
      jpft12@gmail.com.
FAU - Gaspar, J
AU  - Gaspar J
AD  - Faculty of Medical Sciences UNL, Department of Genetics, Lisbon, Portugal.
FAU - Roma-Torres, J
AU  - Roma-Torres J
AD  - National Institute of Health, Centre of Occupational and Environmental Health, 
      Praca Coronel Pacheco, 15, 4050-453 Porto, Portugal.
FAU - Silva, S
AU  - Silva S
AD  - National Institute of Health, Centre of Occupational and Environmental Health, 
      Praca Coronel Pacheco, 15, 4050-453 Porto, Portugal.
FAU - Costa, C
AU  - Costa C
AD  - National Institute of Health, Centre of Occupational and Environmental Health, 
      Praca Coronel Pacheco, 15, 4050-453 Porto, Portugal.
FAU - Roach, J
AU  - Roach J
AD  - Cancer Biomarkers and Prevention Group, University of Leicester, UK.
FAU - Mayan, O
AU  - Mayan O
AD  - National Institute of Health, Centre of Occupational and Environmental Health, 
      Praca Coronel Pacheco, 15, 4050-453 Porto, Portugal.
FAU - Rueff, J
AU  - Rueff J
AD  - Faculty of Medical Sciences UNL, Department of Genetics, Lisbon, Portugal.
FAU - Farmer, P B
AU  - Farmer PB
AD  - Cancer Biomarkers and Prevention Group, University of Leicester, UK.
LA  - eng
GR  - G0100873/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070505
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Air Pollutants, Occupational)
RN  - 0 (Biomarkers)
RN  - 0 (Enzymes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Glyoxylates)
RN  - 0 (Hemoglobins)
RN  - 0 (Mandelic Acids)
RN  - 2PZL5A0W0M (phenylglyoxylic acid)
RN  - 44LJ2U959V (Styrene)
RN  - 9QH06NGT6O (styrene oxide)
RN  - HG18B9YRS7 (Valine)
RN  - NH496X0UJX (mandelic acid)
SB  - IM
MH  - Adult
MH  - Air Pollutants, Occupational/pharmacokinetics/*toxicity
MH  - Biomarkers/analysis/urine
MH  - Chemical Industry
MH  - Enzymes/*genetics
MH  - Epoxy Compounds/*analysis/metabolism
MH  - Female
MH  - Glyoxylates/urine
MH  - Hemoglobins/*analysis/metabolism
MH  - Humans
MH  - Inactivation, Metabolic/genetics
MH  - Male
MH  - Mandelic Acids/urine
MH  - Occupational Exposure/adverse effects/analysis
MH  - *Polymorphism, Genetic
MH  - Styrene/pharmacokinetics/*toxicity
MH  - Valine/*analysis/metabolism
MH  - Workplace/standards
EDAT- 2007/06/15 09:00
MHDA- 2007/09/18 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/02/21 00:00 [received]
PHST- 2007/04/30 00:00 [revised]
PHST- 2007/04/30 00:00 [accepted]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0300-483X(07)00260-0 [pii]
AID - 10.1016/j.tox.2007.04.019 [doi]
PST - ppublish
SO  - Toxicology. 2007 Jul 31;237(1-3):58-64. doi: 10.1016/j.tox.2007.04.019. Epub 2007 
      May 5.