PMID- 17567699
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 17
DP  - 2007 Sep
TI  - Functionally distinct transmission of human immunodeficiency virus type 1 
      mediated by immature and mature dendritic cells.
PG  - 8933-43
AB  - Dendritic cells (DCs) potently stimulate the transmission of human 
      immunodeficiency virus type 1 (HIV-1) to CD4(+) T cells. Immature DCs (iDCs) 
      located in submucosal tissues can capture HIV-1 and migrate to lymphoid tissues, 
      where they become mature DCs (mDCs) for effective antigen presentation. DC 
      maturation promotes HIV-1 transmission; however, the underlying mechanisms remain 
      unclear. Here we have compared monocyte-derived iDCs and mDCs for their 
      efficiencies and mechanisms of HIV-1 transmission. We have found that mDCs 
      significantly facilitate HIV-1 endocytosis and efficiently concentrate HIV-1 at 
      virological synapses, which contributes to mDC-enhanced viral transmission, at 
      least in part. mDCs were more efficient than iDCs in transferring HIV-1 to 
      various types of target cells independently of C-type lectins, which partially 
      accounted for iDC-mediated HIV-1 transmission. Efficient HIV-1 trans-infection 
      mediated by iDCs and mDCs required contact between DCs and target cells. 
      Moreover, rapid HIV-1 degradation occurred in both iDCs and mDCs, which 
      correlated with the lack of HIV-1 retention-mediated long-term viral 
      transmission. Our results provide new insights into the mechanisms underlying 
      DC-mediated HIV-1 transmission, suggesting that HIV-1 exploits mDCs to facilitate 
      its dissemination within lymphoid tissues.
FAU - Wang, Jian-Hua
AU  - Wang JH
AD  - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 
      8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
FAU - Janas, Alicia M
AU  - Janas AM
FAU - Olson, Wendy J
AU  - Olson WJ
FAU - Wu, Li
AU  - Wu L
LA  - eng
GR  - R01 AI068493/AI/NIAID NIH HHS/United States
GR  - R01 AI068493-02/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070613
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Lectins, C-Type)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/ultrastructure/*virology
MH  - Endocytosis
MH  - HIV-1/*growth & development/physiology
MH  - Humans
MH  - Lectins, C-Type/physiology
MH  - Microscopy, Electron, Transmission
MH  - Virus Internalization
PMC - PMC1951429
EDAT- 2007/06/15 09:00
MHDA- 2007/09/29 09:00
PMCR- 2008/01/01
CRDT- 2007/06/15 09:00
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
PHST- 2008/01/01 00:00 [pmc-release]
AID - JVI.00878-07 [pii]
AID - 0878-07 [pii]
AID - 10.1128/JVI.00878-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Sep;81(17):8933-43. doi: 10.1128/JVI.00878-07. Epub 2007 Jun 13.