PMID- 17568134
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20121115
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 366
DP  - 2007
TI  - Adeno-associated viral vector-delivered hypoxia-inducible gene expression in 
      ischemic hearts.
PG  - 331-42
AB  - This chapter describes a system using adeno-associated viral (AAV) vector to 
      deliver hypoxia-inducible gene expression to ischemic hearts. The hypoxia 
      induction of gene expression in this system is based on the accumulation of 
      hypoxia-inducible factor-1 (HIF-1) in ischemic hearts and the use of 
      hypoxia-response element (HRE) identified from the enhancers of genes, the 
      expression of which can be induced by hypoxia. The methods of plasmid and AAV 
      vector construction for hypoxia-inducible gene expression, viral vector 
      production and purification, and viral titer determination are described. This 
      chapter also illustrates the methods that can be used to test hypoxia-inducible 
      gene expression in vitro and in vivo, including hypoxia treatment of cultured 
      cells, generation of murine ischemic heart models, and analysis of gene 
      expression.
FAU - Su, Hua
AU  - Su H
AD  - Cardiovascular Research Institute/Department of Medicine, University of 
      California, San Francisco, USA.
FAU - Kan, Yuet Wai
AU  - Kan YW
LA  - eng
GR  - HL067969/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Dependovirus/*genetics
MH  - Erythropoietin/genetics
MH  - Gene Expression
MH  - Genetic Therapy
MH  - *Genetic Vectors
MH  - HeLa Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics/metabolism
MH  - Lac Operon/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Myocardial Ischemia/genetics/metabolism/*therapy
MH  - Response Elements
MH  - Transcription Factors
MH  - Transfection
MH  - Vascular Endothelial Growth Factors/genetics/metabolism
EDAT- 2007/06/15 09:00
MHDA- 2007/07/13 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - 1-59745-030-8:331 [pii]
AID - 10.1007/978-1-59745-030-0_19 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2007;366:331-42. doi: 10.1007/978-1-59745-030-0_19.