PMID- 17568938
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20210915
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 102
IP  - 3
DP  - 2007 Jun
TI  - Antimalarial drugs disrupt ion homeostasis in malarial parasites.
PG  - 329-34
AB  - Plasmodium chabaudi malaria parasite organelles are major elements for ion 
      homeostasis and cellular signaling and also target for antimalarial drugs. By 
      using confocal imaging of intraerythrocytic parasites we demonstrated that the 
      dye acridine orange (AO) is accumulated into P. chabaudi subcellular 
      compartments. The AO could be released from the parasite organelles by collapsing 
      the pH gradient with the K+/H+ ionophore nigericin (20 microM), or by inhibiting 
      the H+-pump with bafilomycin (4 microM). Similarly, in isolated parasites loaded 
      with calcium indicator Fluo 3-AM, bafilomycin caused calcium mobilization of the 
      acidic calcium pool that could also be release with nigericin. Interestingly 
      after complete release of the acidic compartments, addition of thapsigargin at 10 
      microM was still effective in releasing parasite intracellular calcium stores in 
      parasites at trophozoite stage. The addition of antimalarial drugs chloroquine 
      and artemisinin resulted in AO release from acidic compartments and also affected 
      maintenance of calcium in ER store by using different drug concentrations.
FAU - Gazarini, Marcos L
AU  - Gazarini ML
AD  - Departamento de Fisiologia, Instituto de Biociencias, Universidade de Sao Paulo, 
      Suo Paulo, Brasil. cgarcia@usp.br
FAU - Sigolo, Carlos A O
AU  - Sigolo CA
FAU - Markus, Regina P
AU  - Markus RP
FAU - Thomas, Andrew P
AU  - Thomas AP
FAU - Garcia, Celia R S
AU  - Garcia CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Antimalarials)
RN  - 0 (Artemisinins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Ion Channels)
RN  - 886U3H6UFF (Chloroquine)
RN  - 9RMU91N5K2 (artemisinin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Antimalarials/*pharmacology
MH  - Artemisinins/pharmacology
MH  - Chloroquine/pharmacology
MH  - Erythrocytes/*parasitology
MH  - Female
MH  - Fluorescent Dyes
MH  - Homeostasis/*drug effects
MH  - Hydrogen-Ion Concentration
MH  - Ion Channels/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Confocal
MH  - Plasmodium chabaudi/*drug effects
EDAT- 2007/06/15 09:00
MHDA- 2007/10/25 09:00
CRDT- 2007/06/15 09:00
PHST- 2007/02/08 00:00 [received]
PHST- 2007/06/15 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2007/06/15 09:00 [entrez]
AID - S0074-02762007000300012 [pii]
AID - 10.1590/s0074-02762007000300012 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2007 Jun;102(3):329-34. doi: 
      10.1590/s0074-02762007000300012.