PMID- 17572707 OWN - NLM STAT- MEDLINE DCOM- 20070918 LR - 20191210 IS - 0268-3369 (Print) IS - 0268-3369 (Linking) VI - 40 IP - 4 DP - 2007 Aug TI - Donor-origin cell engraftment after intraosseous or intravenous bone marrow transplantation in a rat model. PG - 373-80 AB - We compared the effects of intraosseous BMT with those of standard i.v. BMT on the efficacy on donor-cell engraftment into the BM and lymphoid organs across an MHC barrier in rats. Twenty-four intraosseous and 24 i.v. BMTs were performed from 48 ACI (RT1(a)) donors to 48 Lewis (RT1(l)) recipients. Each transplant group received either intraosseous or i.v. BMT. Groups I and II served as controls without immunosuppression (n=16); groups III and IV received cyclosporine monotherapy (n=16); and V and VI received alphabeta-TCR monoclonal antibody and cyclosporine A (alphabeta-TCR/CsA) for 7 days (n=16). In each group, four rats received 35 x 10(6) transplanted bone marrow cells (BMCs) and four received 70 x 10(6) cells. All animals survived without GVHD. Mean (+/-s.d.) donor-cell engraftment into BM of recipients after intraosseous BMT was 7.9% (+/-1.3%) in recipients receiving alphabeta-TCR-CsA and 70 x 10(6) BMCs, and 4.2% (+/-1.4%) in recipients after i.v. transplantation. The seeding efficacy of donor cells into lymphoid tissue was greater after intraosseous BMT and alphabeta-TCR-CsA than after standard i.v. transplantation. In our model, intraosseous BMT facilitated donor-cell engraftment under short-term immunodepletive alphabeta-TCR/CsA protocol, which resulted in a temporary state of immune unresponsiveness. FAU - Klimczak, A AU - Klimczak A AD - Department of Plastic Surgery, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA. FAU - Unal, S AU - Unal S FAU - Jankowska, A AU - Jankowska A FAU - Coburn, C AU - Coburn C FAU - Siemionow, M AU - Siemionow M LA - eng PT - Evaluation Study PT - Journal Article DEP - 20070618 PL - England TA - Bone Marrow Transplant JT - Bone marrow transplantation JID - 8702459 SB - IM MH - Animals MH - Bone Marrow Transplantation/*methods/physiology MH - Disease Models, Animal MH - *Graft Survival MH - Infusions, Intraosseous MH - Rats MH - Rats, Inbred Lew MH - *Transplantation Chimera MH - Transplantation Conditioning/methods EDAT- 2007/06/19 09:00 MHDA- 2007/09/19 09:00 CRDT- 2007/06/19 09:00 PHST- 2007/06/19 09:00 [pubmed] PHST- 2007/09/19 09:00 [medline] PHST- 2007/06/19 09:00 [entrez] AID - 1705743 [pii] AID - 10.1038/sj.bmt.1705743 [doi] PST - ppublish SO - Bone Marrow Transplant. 2007 Aug;40(4):373-80. doi: 10.1038/sj.bmt.1705743. Epub 2007 Jun 18.