PMID- 17573666
OWN - NLM
STAT- MEDLINE
DCOM- 20080115
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 27
IP  - 9
DP  - 2007 Sep
TI  - Development of noninvasive fetal DNA diagnosis from nucleated erythrocytes 
      circulating in maternal blood.
PG  - 846-8
AB  - BACKGROUND: A considerable effort is being spent in developing noninvasive 
      prenatal DNA diagnostic procedures. We recently reported that nucleated 
      erythrocytes (NRBCs) can be enriched from maternal blood by a galactose-specific 
      lectin method. In the present study, to prove that fetal NRBCs are definitely 
      present in maternal blood and are a good source for fetal genetic diagnosis, we 
      evaluated methods for lectin enrichment and subsequent fluorescence in situ 
      hybridization (FISH) analysis through fetal gender determination. METHODS: 
      Peripheral blood samples were collected from pregnant women (median 15, range: 
      10-18 weeks). From the blood samples, NRBCs were enriched based on 
      galactose-specific lectin method. After detecting them by their morphology, NRBCs 
      are separated and taken in a new glass slide by micromanipulator. We analyzed 
      fetal gender using X and Y-chromosome-specific FISH probes. The results were 
      compared with fetal gender analysis using Y-chromosomal sequences in maternal 
      plasma. RESULTS: The fetal gender analyses by FISH in 20 pregnant women were all 
      in accordance with the results from maternal plasma analyses. It is confirmed 
      that fetal NRBCs were present in maternal blood and that 30.4% of NRBCs in 
      maternal blood were fetal in origin. CONCLUSION: We have successfully carried out 
      a noninvasive prenatal DNA diagnosis of fetal gender by using galactose-specific 
      lectin method and subsequent FISH analysis.
CI  - 2007 John Wiley & Sons, Ltd
FAU - Sekizawa, Akihiko
AU  - Sekizawa A
AD  - The Study Group for Fetal DNA Diagnosis from Maternal Blood, Japan. 
      sekizawa@med.showa-u.ac.jp
FAU - Purwosunu, Yuditiya
AU  - Purwosunu Y
FAU - Farina, Antonio
AU  - Farina A
FAU - Okai, Takashi
AU  - Okai T
FAU - Takabayashi, Haruo
AU  - Takabayashi H
FAU - Kita, Mikiko
AU  - Kita M
FAU - Yura, Hirofumi
AU  - Yura H
FAU - Kitagawa, Michihiro
AU  - Kitagawa M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/analysis/*isolation & purification/metabolism
MH  - Erythroblasts/cytology/*metabolism
MH  - Female
MH  - *Fetus/metabolism
MH  - Humans
MH  - Male
MH  - Maternal-Fetal Relations
MH  - *Mothers
MH  - Pregnancy
MH  - Pregnancy Trimester, First/blood
MH  - Pregnancy Trimester, Second/blood
MH  - Prenatal Diagnosis/*methods
EDAT- 2007/06/19 09:00
MHDA- 2008/01/16 09:00
CRDT- 2007/06/19 09:00
PHST- 2007/06/19 09:00 [pubmed]
PHST- 2008/01/16 09:00 [medline]
PHST- 2007/06/19 09:00 [entrez]
AID - 10.1002/pd.1792 [doi]
PST - ppublish
SO  - Prenat Diagn. 2007 Sep;27(9):846-8. doi: 10.1002/pd.1792.