PMID- 17574967
OWN - NLM
STAT- MEDLINE
DCOM- 20070815
LR  - 20070618
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 176
IP  - 1
DP  - 2007 Jul 1
TI  - Molecular cytogenetic findings in a four-way t(1;12;5;12)(p36;p13;q33;q24) 
      underlying the ETV6-PDGFRB fusion gene in chronic myelomonocytic leukemia.
PG  - 67-71
AB  - Fluorescence in situ hybridization (FISH) and reverse-transcription polymerase 
      chain reaction (RT-PCR) detected the ETV6-PDGFRB fusion in a patient with chronic 
      myelomonocytic leukemia characterized by bone marrow and peripheral blood 
      eosinophilia and a four-way t(1;12;5;12)(p36;p13;q33;q24) on bone marrow cells. 
      The patient consequently underwent imatinib mesylate therapy and achieved 
      hematologic, FISH, and molecular remission. The FICTION technique (fluorescence 
      immunophenotyping and interphase cytogenetics as a tool for the investigation of 
      neoplasms) demonstrated that eosinophils and CD13(+) and CD14(+) cells belong to 
      the neoplastic clone bearing the ETV6-PDGFRB rearrangement. Molecular 
      cytogenetics is the most reliable approach to detect the involvement of 
      promiscuous genes, such as PDGFRB, and to properly classify genetic entities for 
      which targeted therapies are available. Assessment of cell lineages harboring the 
      genomic lesion may contribute in the understanding of leukemogenic pathways.
FAU - Crescenzi, Barbara
AU  - Crescenzi B
AD  - Department of Hematology, University of Perugia, IBiT Foundation (Fondazione 
      IRCCS Biotecnologie nel Trapianto), via Brunamonti 51, 06122 Perugia, Italy.
FAU - La Starza, Roberta
AU  - La Starza R
FAU - Nozzoli, Chiara
AU  - Nozzoli C
FAU - Ciolli, Stefania
AU  - Ciolli S
FAU - Matteucci, Caterina
AU  - Matteucci C
FAU - Romoli, Silvia
AU  - Romoli S
FAU - Rigacci, Luigi
AU  - Rigacci L
FAU - Gorello, Paolo
AU  - Gorello P
FAU - Bosi, Alberto
AU  - Bosi A
FAU - Martelli, Massimo F
AU  - Martelli MF
FAU - Marynen, Peter
AU  - Marynen P
FAU - Mecucci, Cristina
AU  - Mecucci C
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (ETV6-PDGFRB fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 5
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelomonocytic, Chronic/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - *Translocation, Genetic
EDAT- 2007/06/19 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/06/19 09:00
PHST- 2007/01/19 00:00 [received]
PHST- 2007/03/04 00:00 [revised]
PHST- 2007/03/09 00:00 [accepted]
PHST- 2007/06/19 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/06/19 09:00 [entrez]
AID - S0165-4608(07)00142-2 [pii]
AID - 10.1016/j.cancergencyto.2007.03.004 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Jul 1;176(1):67-71. doi: 
      10.1016/j.cancergencyto.2007.03.004.