PMID- 17575266
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 82
IP  - 3
DP  - 2007 Sep
TI  - The extra domain A of fibronectin stimulates murine mast cells via toll-like 
      receptor 4.
PG  - 657-65
AB  - The activation of mast cells by extra domain A of fibronectin (FN-EDA), an 
      endogenous ligand of TLR4, and its contribution to the pathogenesis of rheumatoid 
      arthritis (RA) in vivo were examined. FN-EDA, but no other domain of the 
      fibronectin fragment, III(11) (FN-III(11)) and III(12) (FN-III(12)), stimulated 
      bone marrow-derived murine mast cells (BMMCs) dose-dependently to secret 
      cytokines (TNF-alpha, IL-6, and IL-1beta), similar to the pattern produced by 
      LPS. FN-EDA-induced cytokine production was mediated by TLR4, as cytokine 
      production by FN-EDA was absent in TLR4-deficient (TLR4-/-) BMMCs. We examined 
      the roles of TLR4-mediated mast cell activation by this form of fibronectin 
      fragment in the pathogenesis of RA in vivo. The injection of FN-EDA, but not 
      FN-III(11)and FN-III(12), to joints resulted in joint swelling of mice in vivo. 
      Genetically mast cell-deficient WBB6F(1)-W/W(v) mice exhibited significantly less 
      swelling and cytokine production compared with mast cell-sufficient +/+ mice, 
      suggesting that swelling and inflammatory cytokine production were partially 
      dependent on tissue mast cells. Reduced swelling and cytokine production were 
      recovered by the reconstitution of tissue mast cells by the injection of BMMCs 
      from wild-type mice but not from TLR4-/- mice. Altogether, these results suggest 
      that the TLR4-mediated activation of mast cells by endogenous ligand FN-EDA might 
      contribute to the pathogenesis of RA through proinflammatory cytokine production.
FAU - Gondokaryono, Srie Prihianti
AU  - Gondokaryono SP
AD  - Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Ushio, Hiroko
AU  - Ushio H
FAU - Niyonsaba, Francois
AU  - Niyonsaba F
FAU - Hara, Mutsuko
AU  - Hara M
FAU - Takenaka, Hiroshi
AU  - Takenaka H
FAU - Jayawardana, Sumanasiri T M
AU  - Jayawardana ST
FAU - Ikeda, Shigaku
AU  - Ikeda S
FAU - Okumura, Ko
AU  - Okumura K
FAU - Ogawa, Hideoki
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070615
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/etiology/metabolism/pathology
MH  - Blotting, Western
MH  - Bone Marrow/metabolism/pathology
MH  - Fibronectins/genetics/*pharmacology
MH  - Inflammation/etiology/pathology
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Joints/metabolism/pathology
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - NF-kappa B/metabolism
MH  - Protein Structure, Tertiary
MH  - Recombinant Proteins/metabolism
MH  - Toll-Like Receptor 2/metabolism
MH  - Toll-Like Receptor 4/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - beta-N-Acetylhexosaminidases/metabolism
EDAT- 2007/06/19 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/06/19 09:00
PHST- 2007/06/19 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/06/19 09:00 [entrez]
AID - jlb.1206730 [pii]
AID - 10.1189/jlb.1206730 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2007 Sep;82(3):657-65. doi: 10.1189/jlb.1206730. Epub 2007 Jun 15.