PMID- 17576238
OWN - NLM
STAT- MEDLINE
DCOM- 20070831
LR  - 20070619
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 16
IP  - 7
DP  - 2007 Jul
TI  - Switch in syndecan-1 and syndecan-4 expression controls maturation associated 
      dendritic cell motility.
PG  - 580-9
AB  - Dendritic cells (DCs) need to mobilize within the extracellular matrix (ECM) 
      during their maturation and concomitant migration from peripheral sites to 
      lymphoid organs. Syndecans are cell surface proteoglycans that mediate the 
      interaction of DCs with the ECM. Here we investigated the influence of syndecans 
      on dendritic cell motility and morphology. Langerhans cells of the epidermis and 
      monocyte-derived DCs were found to undergo a switch in syndecan expression during 
      maturation. Syndecan-1 was downregulated and syndecan-4 was strongly upregulated 
      within the first hours of lipopolysaccharide-induced dendritic cell maturation 
      and during Langerhans cell emigration from human skin, as shown by flow cytometry 
      and qRT-PCR. Syndecan-1 downregulation was inhibited by syndecan-4 siRNA 
      knock-down, indicating a functional interconnection between enhanced syndecan-4 
      expression and syndecan-1 downregulation. Syndecan-4 upregulation is functionally 
      involved in dendritic cell motility, as inhibition of syndecan-4 function by 
      means of blocking antibodies or through siRNA knock-down decreased dendritic cell 
      motility. In other experiments, the cytoskeletal component a-actinin was observed 
      to be upregulated in DCs as a consequence of the induction of maturation, and was 
      found to colocalize with syndecan-4. Furthermore, lammellopodial spreading by DCs 
      on fibronectin (FN)-coated surfaces was dependent on syndecan-4. This binding of 
      syndecan-4 to FN and its association with the cytoskeleton may be relevant for 
      syndecan-4-dependent dendritic cell motility. We conclude that the switch in 
      syndecan expression during dendritic cell maturation controls the motility of DCs 
      in a way that appears to be crucial for their mobilization from peripheral sites 
      and subsequent migration to lymphoid tissues.
FAU - Averbeck, Marco
AU  - Averbeck M
AD  - Department of Dermatology, Venerology and Allergology, Leipzig University, 
      Leipzig, Germany. marco.averbeck@medizin.uni-leipzig.de
FAU - Gebhardt, Carl
AU  - Gebhardt C
FAU - Anderegg, Ulf
AU  - Anderegg U
FAU - Termeer, Christian
AU  - Termeer C
FAU - Sleeman, Jonathan P
AU  - Sleeman JP
FAU - Simon, Jan C
AU  - Simon JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Microfilament Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SDC1 protein, human)
RN  - 0 (SDC4 protein, human)
RN  - 0 (Syndecan-1)
RN  - 0 (Syndecan-4)
RN  - 146808-54-0 (fascin)
SB  - IM
MH  - Actins/metabolism
MH  - Carrier Proteins/metabolism
MH  - Cell Differentiation/physiology
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism/physiology
MH  - Fibronectins/physiology
MH  - Humans
MH  - Infant, Newborn
MH  - Langerhans Cells/*metabolism/physiology
MH  - Lipopolysaccharides
MH  - Microfilament Proteins/metabolism
MH  - Microscopy, Confocal
MH  - RNA, Small Interfering
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Syndecan-1/*metabolism
MH  - Syndecan-4/*metabolism
EDAT- 2007/06/20 09:00
MHDA- 2007/09/01 09:00
CRDT- 2007/06/20 09:00
PHST- 2007/06/20 09:00 [pubmed]
PHST- 2007/09/01 09:00 [medline]
PHST- 2007/06/20 09:00 [entrez]
AID - EXD568 [pii]
AID - 10.1111/j.1600-0625.2007.00568.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2007 Jul;16(7):580-9. doi: 10.1111/j.1600-0625.2007.00568.x.