PMID- 17576695
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20220318
IS  - 1462-0324 (Print)
IS  - 1462-0324 (Linking)
VI  - 46
IP  - 9
DP  - 2007 Sep
TI  - Characterization of haemorrhagic pulmonary capillaritis: another manifestation of 
      Pristane-induced lupus.
PG  - 1405-10
AB  - OBJECTIVES: Pristane-induced lupus is a well-established model of murine lupus. 
      Mice injected with Pristane develop lupus-specific autoantibodies and 
      glomerulonephritis. A chance observation led us to identify and characterize 
      haemorrhagic pulmonary capillaritis in Pristane-injected mice. METHODS: 
      Eight-week-old C57Bl/10 (B10, H-2(b)) mice received a single intraperitoneal 
      injection of 0.5 ml of Pristane. Control mice received phosphate-buffered saline 
      (PBS) injection. Mice were bled at 2 weeks after Pristane injection and monthly 
      thereafter for serology and for antinuclear antibody (ANA). To characterize 
      pulmonary disease, bronchoalveolar lavage (BAL) was carried out for total and 
      differential cell count. Cytokines levels were checked for IL-2, IL-4, TNF-alpha, 
      IFN-gamma, IL-6 and IL-10. Lungs were examined by histopathology and electron 
      microscopy. RESULTS: All mice injected with Pristane developed a pulmonary 
      capillaritis with perivascular infiltration with macrophages, neutrophils, 
      lymphocytes and eosinophils. In addition, alveoli showed macrophage and 
      neutrophil infiltration. The degree of perivascular and alveolar inflammation was 
      moderate to severe. BAL was inflammatory with cell composition of macrophages, 
      neutrophils, lymphocyte and eosinophils. There was evidence of endothelial injury 
      on electron microscopy but no evidence of immune complex deposition. IL-6 and 
      IL-10 were increased in BAL but levels of TNF-alpha, IFN-gamma, IL-2 and IL-4 
      were not. Anti-neutrophil cytoplasm antibody (ANCA) was negative. Kidneys 
      demonstrated an increase in mesangial matrix and cellularity compatible with WHO 
      Class II lupus lesion. There were immune complexes and complement deposition in 
      the kidney. There were oil granulomas in peritoneum, spleen and liver but no 
      evidence of vasculitis in these organs was seen. CONCLUSION: The relative ease 
      and high penetrability of lesion makes it an attractive model to study pulmonary 
      vasculitis.
FAU - Chowdhary, V R
AU  - Chowdhary VR
AD  - Division of Rheumatology, Department of Medicine, Mayo Clinic College of 
      Medicine, 200, 1st SW Rochester, MN 55905, USA.
FAU - Grande, J P
AU  - Grande JP
FAU - Luthra, H S
AU  - Luthra HS
FAU - David, C S
AU  - David CS
LA  - eng
PT  - Journal Article
DEP - 20070618
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Terpenes)
RN  - 130068-27-8 (Interleukin-10)
RN  - 26HZV48DT1 (pristane)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/biosynthesis
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Disease Models, Animal
MH  - Granuloma, Foreign-Body/chemically induced/pathology
MH  - Hemorrhage/*chemically induced/immunology/pathology
MH  - Immunosuppressive Agents/toxicity
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Lung/ultrastructure
MH  - Lung Diseases/*chemically induced/immunology/pathology
MH  - Lupus Erythematosus, Systemic/*chemically induced/immunology/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Terpenes/*toxicity
MH  - Vasculitis/*chemically induced/immunology/pathology
EDAT- 2007/06/20 09:00
MHDA- 2007/10/25 09:00
CRDT- 2007/06/20 09:00
PHST- 2007/06/20 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2007/06/20 09:00 [entrez]
AID - kem117 [pii]
AID - 10.1093/rheumatology/kem117 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2007 Sep;46(9):1405-10. doi: 10.1093/rheumatology/kem117. 
      Epub 2007 Jun 18.