PMID- 17577055
OWN - NLM
STAT- MEDLINE
DCOM- 20070827
LR  - 20220331
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 56
IP  - Pt 7
DP  - 2007 Jul
TI  - Peptide nucleic acid fluorescence in situ hybridization for rapid detection of 
      Klebsiella pneumoniae from positive blood cultures.
PG  - 914-917
LID - 10.1099/jmm.0.46829-0 [doi]
AB  - This study evaluated a novel peptide nucleic acid (PNA) probe targeting a region 
      of the 23S rRNA gene of Klebsiella pneumoniae by fluorescence in situ 
      hybridization (FISH). Analytical performance was determined using 39 reference 
      strains and other well-characterized strains of Klebsiella spp. and Enterobacter 
      aerogenes. The probe was found to be specific for the K. pneumoniae complex (K. 
      pneumoniae including Klebsiella ozaenae and Klebsiella variicola). The diagnostic 
      accuracy was evaluated with 264 blood cultures containing Gram-negative rods. 
      Using conventional identification as the reference, performance specifications 
      were as follows: sensitivity 98.8 %, specificity 99.5 %, positive predictive 
      value 98.8 % and negative predictive value 99.5 %. Discrepancies were resolved by 
      PNA FISH retest and phenotypic tests. In conclusion, the K. pneumoniae probe 
      provided an accurate diagnosis within 3 h and may supplement other methods for 
      direct identification of Gram-negative bacteria.
FAU - Sogaard, Mette
AU  - Sogaard M
AD  - Department of Clinical Epidemiology, Aalborg Hospital, Aarhus University 
      Hospital, Aarhus, Denmark.
AD  - Department of Clinical Microbiology, Aalborg Hospital, Aarhus University 
      Hospital, Postbox 365, 9100 Aalborg, Denmark.
FAU - Hansen, Dennis S
AU  - Hansen DS
AD  - Department of Clinical Microbiology, Hillerod Sygehus, Denmark.
AD  - The International Escherichia and Klebsiella Reference Center (WHO), Statens 
      Serum Institut, Copenhagen, Denmark.
FAU - Fiandaca, Mark J
AU  - Fiandaca MJ
AD  - AdvanDx Inc., Woburn, MA, USA.
FAU - Stender, Henrik
AU  - Stender H
AD  - AdvanDx Inc., Woburn, MA, USA.
FAU - Schonheyder, Henrik C
AU  - Schonheyder HC
AD  - Department of Clinical Microbiology, Aalborg Hospital, Aarhus University 
      Hospital, Postbox 365, 9100 Aalborg, Denmark.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Culture Media)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Bacteremia/microbiology
MH  - *Bacterial Typing Techniques
MH  - Bacteriological Techniques
MH  - Blood/*microbiology
MH  - Culture Media
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Klebsiella Infections/*microbiology
MH  - Klebsiella pneumoniae/classification/genetics/*isolation & purification
MH  - Nucleic Acid Probes/*genetics
MH  - Peptide Nucleic Acids/*genetics
MH  - Time Factors
EDAT- 2007/06/20 09:00
MHDA- 2007/08/28 09:00
CRDT- 2007/06/20 09:00
PHST- 2007/06/20 09:00 [pubmed]
PHST- 2007/08/28 09:00 [medline]
PHST- 2007/06/20 09:00 [entrez]
AID - 10.1099/jmm.0.46829-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2007 Jul;56(Pt 7):914-917. doi: 10.1099/jmm.0.46829-0.