PMID- 1757742 OWN - NLM STAT- MEDLINE DCOM- 19920206 LR - 20191111 IS - 0740-7769 (Print) IS - 0740-7769 (Linking) VI - 8 IP - 5 DP - 1991 Oct TI - Scanning electron microscope (SEM) study of mouse embryos obtained from isolated blastomeres. PG - 279-85 AB - Preimplantation diagnosis and embryo sexing offer great possibilities in the prevention of human diseases and in the field of animal production. These techniques involve blastomere isolation. Isolated blastomeres can grow in culture and develop as whole embryos. In this paper we describe, at the scanning electron microscope level, the characteristics of the plasma membrane surface of isolated blastomeres obtained from mouse embryos at the two-cell stage and of embryos grown to the 2/4- and 4/8-cell stages and compare them to control embryos grown in vitro. According to our results the in vitro manipulation of these embryos does not affect the surface characteristics of the plasma membrane in the early cleavage stages. FAU - Ponsa, M AU - Ponsa M AD - Departament de Biologia Cellular i Fisiologia, Institut de Biologia Fonamental Vicent Villar Palasi, Universitat Autonoma de Barcelona, Spain. FAU - Nogues, C AU - Nogues C FAU - Vidal, F AU - Vidal F FAU - Egozcue, J AU - Egozcue J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J In Vitro Fert Embryo Transf JT - Journal of in vitro fertilization and embryo transfer : IVF JID - 8412594 SB - IM MH - Animals MH - Blastomeres/physiology/*ultrastructure MH - Cell Communication/physiology MH - Cell Membrane/ultrastructure MH - Embryo, Mammalian/physiology/*ultrastructure MH - Female MH - Mice MH - Microscopy, Electron, Scanning MH - Microvilli/ultrastructure MH - Pregnancy EDAT- 1991/10/01 00:00 MHDA- 1991/10/01 00:01 CRDT- 1991/10/01 00:00 PHST- 1991/10/01 00:00 [pubmed] PHST- 1991/10/01 00:01 [medline] PHST- 1991/10/01 00:00 [entrez] AID - 10.1007/BF01139785 [doi] PST - ppublish SO - J In Vitro Fert Embryo Transf. 1991 Oct;8(5):279-85. doi: 10.1007/BF01139785.