PMID- 17578288
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20070620
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 21
IP  - 5
DP  - 2007 May
TI  - [Experimental study on transplantation of embryonic stem cells in treating spinal 
      cord injury].
PG  - 487-91
AB  - OBJECTIVE: To observe the effect of transplantation of embryonic stem cell (ES) 
      on neurological functional recovery of injured spinal cord in adult mouse. 
      METHODS: The ES cells were cultured and induced in vitro. Fifty C57/BL6J mice 
      were made animal model of semi-cut mice of T9,10. The ES cell-derived neural 
      precursors cells were transplanted into the vertebral canal around injured spinal 
      cord semi-cut mice. Twenty-eight C57/BL6J mice were randomly divided into three 
      groups: sham operation group(group A, n=9), operation/cell group (group B, n = 
      10), and operation/DMEM group (group C, n = 9). RT-PCR analysis, X-gal staining 
      and immunofluorescence were used to observe the cells survival and 
      differentiation in the spinal crod. BBB test was performed to study functional 
      improvement. RESULTS: ES cells induced and cultured in vitro displayed clonal 
      growth with circle or ovoid shape and had one or more nucleoli. RT-PCR result 
      showed that the induced ES cells expressed mRNA of Nestin and 
      microtubule-associated protein, but did not express glial fibrillory acidic 
      protein (GFAP). There was statistically significant difference in BBB scoring 
      between group A and groups B, C after operation (P <0.01). There was 
      statistically significant difference in BBB scoring at 1, 2 and 4 weeks of 
      operation (P < 0.01), but no statistically significant difference at 6 and 8 
      weeks of operation between groups B and C (P>0.05). The X-gal staining results 
      were positive in group B and negative in groups A and C. The immunoflurescence 
      result showed neurofilament green fluor and no expression of GFAP in injured 
      spinal cord region. CONCLUSION: After transplantation, ES cell-derived cells can 
      survive, transfer into the injury position, and differentiate into neurons, but 
      spinal cord function has no obvious improvement.
FAU - Yang, Jianhua
AU  - Yang J
AD  - Orthopedics Department, the First Affiliated Hospital, Jiamusi University, 
      Jiamusi Heilongjiang, 154002, P. R. China. jianhua01@163.com
FAU - Li, Changde
AU  - Li C
FAU - Zhai, Raosheng
AU  - Zhai R
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Transplantation/methods
MH  - Cells, Cultured
MH  - Embryonic Stem Cells/*cytology
MH  - Female
MH  - Hindlimb/physiopathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microtubule-Associated Proteins/biosynthesis
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Neurons/*cytology/metabolism
MH  - Random Allocation
MH  - Recovery of Function
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Cord Injuries/pathology/physiopathology/*surgery
MH  - *Stem Cell Transplantation
EDAT- 2007/06/21 09:00
MHDA- 2009/05/08 09:00
CRDT- 2007/06/21 09:00
PHST- 2007/06/21 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2007/06/21 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 May;21(5):487-91.