PMID- 17580322
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20070924
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 83
IP  - 2
DP  - 2007 Nov
TI  - Influence of the surface structure of a multiblock copolymer on the cellular 
      behavior of primary cell cultures of the upper aerodigestive tract in vitro.
PG  - 558-69
AB  - The influence of the surface topography of a biodegradable copolymer on adhesion, 
      proliferation, and cellular activity of primary cell cultures of the upper 
      aerodigestive tract (ADT) was investigated. On the basis of the important 
      functions of matrix metalloproteinases (MMPs) and their endogenous inhibitors, 
      tissue inhibitor of MMPs (TIMPs) in regulating extracellular matrix remodeling, 
      cellular adhesion and growth, the appearance and kinetics of these enzymes were 
      investigated in primary cells of the upper ADT seeded on different surfaces of a 
      polymeric biomaterial. Primary cell cultures of the upper ADT of Sprague-Dawley 
      rats were seeded on different surfaces (smooth versus rough surface) of a 
      biodegradable multiblock copolymer and on polystyrene surface as control. 
      Conditioned media of the primary cells were analyzed for MMPs and TIMPs by both 
      zymography and radiometric enzyme assay. Cell adhesion and proliferation as well 
      as the kinetics of appearance and activity level of MMP-1, MMP-2, and TIMPs were 
      significantly different depending on the cell type and the surface structure of 
      the multiblock copolymer. In this study, the data obtained indicated that surface 
      topography governed the biological response to biomaterials. Knowledge as to how 
      cells interact with the interface of biomaterials will be necessary in order to 
      eventually design the "ideal" surface of biomaterials, which will be both tissue 
      and organ-optimized in order to best provide clinicians with specific and viable 
      novel therapeutical options in medicine.
CI  - Copyright (c) 2007 Wiley Periodicals, Inc.
FAU - Rickert, Dorothee
AU  - Rickert D
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, University of Ulm, 
      Frauensteige 12, 89075 Ulm, Germany, and Department of Surgery, Children's 
      Hospital, Boston, MA 02115, USA. dorothee.rickert@uniklinik-ulm.de
FAU - Franke, R-P
AU  - Franke RP
FAU - Lendlein, A
AU  - Lendlein A
FAU - Kelch, S
AU  - Kelch S
FAU - Moses, M A
AU  - Moses MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Gastrointestinal Tract/*cytology/enzymology
MH  - Gene Expression Regulation, Enzymologic
MH  - Kinetics
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Mouth/cytology/enzymology
MH  - Polymers/*chemistry/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Respiratory System/*cytology/enzymology
MH  - Surface Properties
MH  - Tissue Inhibitor of Metalloproteinases/genetics/metabolism
EDAT- 2007/06/21 09:00
MHDA- 2007/12/15 09:00
CRDT- 2007/06/21 09:00
PHST- 2007/06/21 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2007/06/21 09:00 [entrez]
AID - 10.1002/jbm.a.31250 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2007 Nov;83(2):558-69. doi: 10.1002/jbm.a.31250.