PMID- 17580689
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20161124
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 2
DP  - 2007 Mar
TI  - [Determination of five 4-hydroxycoumarin rodenticides in whole blood by high 
      performance liquid chromatography with fluorescence detection].
PG  - 214-6
AB  - A simple, accurate and sensitive method has been developed for the simultaneous 
      determination of warfarin, coumatetralyl, bromadiolone, flocoumafen and 
      brodifacoum in whole blood by high performance liquid chromatography (HPLC) with 
      fluorescence detection. The five 4-hydroxycoumarin rodenticides in whole blood 
      were extracted by ethyl acetate, separated on XDB C,, column( 150 mm x 2. 1 mm, 5 
      [microm) by using the mobile phase consisting of methanol-0. 2% acetic acid 
      aqueous solution (88: 12, v/v) at a flow rate of 0. 5 mL/min and detected with a 
      variational time program for fluorescence wavelength. Each analyte was 
      qualitatively determined with its fluorescence excitation spectrum, fluorescence 
      emission spectrum and retention time being compared with those of the reference 
      standard, and quantified with external calibration method. The linear range was 
      0. 01 - 10. 00 mg/L and the limit of quantification was 0. 01 mg/L except 
      warfarin of which the corresponding results were 0. 05 - 10. 00 mg/L and 0. 05 
      mg/L. The recoveries were between 81% and 98% and the relative standard 
      deviations (RSDs) were between 3. 8% and 8. 5%. This method can be used in the 
      diagnosis of the clinical poisoned patients.
FAU - Jin, Micong
AU  - Jin M
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China. 
      jinmc@nbcdc.org.cn
FAU - Chen, Xiaohong
AU  - Chen X
FAU - Li, Xiaoping
AU  - Li X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (4-Hydroxycoumarins)
RN  - 0 (Rodenticides)
RN  - 2Z80062XQ4 (flocoumafen)
RN  - 5Q7ZVV76EI (Warfarin)
RN  - A25P3CP5S7 (bromfenacoum)
RN  - J2FR050NM5 (bromadiolone)
RN  - NWK4HW86A8 (coumatetralyl)
SB  - IM
MH  - 4-Hydroxycoumarins/*blood
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Reproducibility of Results
MH  - Rodenticides/*blood
MH  - Warfarin/blood
EDAT- 2007/06/22 09:00
MHDA- 2010/06/16 06:00
CRDT- 2007/06/22 09:00
PHST- 2007/06/22 09:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2007/06/22 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Mar;25(2):214-6.