PMID- 17580690
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 2
DP  - 2007 Mar
TI  - [Determination of geniposidic acid and chlorogenic acid in male flowers and 
      related products of Eucommia ulmoides by reversed-phase high performance liquid 
      chromatography].
PG  - 217-20
AB  - A simple and rapid high performance liquid chromatographic method has been 
      developed for the determination of geniposidic acid and chlorogenic acid in the 
      male flowers and related products of Eucommia ulmoides. Two components were 
      separated by a Shim-pack VP-ODS column (150 mm x4.6 mm, 5 [microm) with a mobile 
      phase of methanol-water-acetic acid (24 :75: 1, v/v) at a flow rate of 1 mL/min, 
      column temperature of 30 93 and detection wavelength of 240 nm. Under the 
      chromatographic conditions mentioned above, the method performance, such as the 
      number of theoretical plate, resolution, trailing etc have all reached required 
      level. The linear ranges were 0. 025 - 0. 400 g/L for geniposidic acid and 0. 075 
      - 1. 200 g/L for chlorogenic acid, with the correlation coefficients of 0. 999 7 
      and 0. 999 9, respectively. The average recoveries were 100. 2% and 100. 5%, and 
      the relative standard deviations (RSDs) were 1. 47% and 1. 49% respectively. The 
      minimum detection limits were 0. 02 microg/L for geniposidic acid, and 0. 06 
      microg/L for chlorogenic acid. The method developed has demonstrated the 
      characteristics of simple mobile phase composition, short retention, good 
      resolution, high repeatability and precision. It is suitable for the 
      determination of the two compounds in the male flowers of E. ulmoides and related 
      products.
FAU - Dong, Juane
AU  - Dong J
AD  - Northwest A &F University, the Key Laboratory of Exploitation and Utilization of 
      Plant Resources in Shaanxi Province, Yangling 712100, China. dje009@126.com
FAU - Ma, Xihan
AU  - Ma X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Glucosides)
RN  - 0 (Iridoid Glucosides)
RN  - 0 (Iridoids)
RN  - 27741-01-1 (geniposidic acid)
RN  - 318ADP12RI (Chlorogenic Acid)
SB  - IM
MH  - Chlorogenic Acid/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Eucommiaceae/*chemistry
MH  - Flowers/*chemistry
MH  - Glucosides/*analysis
MH  - Iridoid Glucosides
MH  - Iridoids/*analysis
MH  - Reproducibility of Results
EDAT- 2007/06/22 09:00
MHDA- 2010/06/16 06:00
CRDT- 2007/06/22 09:00
PHST- 2007/06/22 09:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2007/06/22 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Mar;25(2):217-20.