PMID- 17581203
OWN - NLM
STAT- MEDLINE
DCOM- 20071011
LR  - 20121115
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 37
IP  - 7
DP  - 2007 Jul
TI  - Inhibitory effects of suplatast tosilate on the differentiation and function of 
      monocyte-derived dendritic cells from patients with asthma.
PG  - 1083-9
AB  - BACKGROUND: Dendritic cells (DCs) are antigen-presenting cells that efficiently 
      activate T cells. OBJECTIVE: We examined the effects of suplatast tosilate, which 
      prevents T-helper type 2 responses, on the differentiation and function of 
      monocyte-derived DCs (moDCs). METHODS: DCs were differentiated in vitro from 
      peripheral monocytes from patients with asthma by the addition of granulocyte 
      macrophage colony-stimulating factor and IL-4 in the presence or absence of 
      suplatast tosilate. Cell surface molecules (CD1a, CD14, CD80, CD83, CD86, HLA-DR) 
      on immature and mature DCs were analysed with flow cytometry, and the secretion 
      of CC chemokine ligand (CCL)17 (thymus and activation-regulated chemokine), 
      IL-12p70, IL-12p40, and IL-10 was measured with an ELISA. We also studied the 
      proliferative responses of allogeneic CD4(+) T cells from healthy subjects to DCs 
      differentiated in the presence of suplatast tosilate. In addition, the production 
      of IFN-gamma and IL-5 by CD4(+) T cells after coculture with untreated DCs or 
      suplatast tosilate-treated DCs was measured with ELISA. RESULTS: Suplatast 
      tosilate significantly inhibited the expression of CD1a, CD80, and CD86 on 
      immature DCs and of CD1a, CD80, CD83, and CD86 on mature DCs. Suplatast tosilate 
      also significantly inhibited the secretion of CCL17, IL-12p70, and IL-12p40; 
      however, the secretion of IL-10 was not affected. The proliferative responses of 
      allogeneic CD4(+) T cells to suplatast tosilate-treated DCs were suppressed. 
      Moreover, suplatast tosilate-treated DCs had an impaired capacity to stimulate 
      CD4(+) T cells to produce IFN-gamma and IL-5. CONCLUSION: Suplatast tosilate 
      inhibits the differentiation, maturation, and function of moDCs.
FAU - Tanaka, A
AU  - Tanaka A
AD  - First Department of Internal Medicine, Showa University School of Medicine, 
      Tokyo, Japan.
FAU - Minoguchi, K
AU  - Minoguchi K
FAU - Samson, K T R
AU  - Samson KT
FAU - Oda, N
AU  - Oda N
FAU - Yokoe, T
AU  - Yokoe T
FAU - Tazaki, T
AU  - Tazaki T
FAU - Yamamoto, Y
AU  - Yamamoto Y
FAU - Yamamoto, M
AU  - Yamamoto M
FAU - Ohta, S
AU  - Ohta S
FAU - Adachi, M
AU  - Adachi M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Antigens, CD)
RN  - 0 (Arylsulfonates)
RN  - 0 (CCL17 protein, human)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokines, CC)
RN  - 0 (IL10 protein, human)
RN  - 0 (IL5 protein, human)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Interleukin-5)
RN  - 0 (Sulfonium Compounds)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - C9J89787U1 (suplatast tosilate)
SB  - IM
CIN - Clin Exp Allergy. 2007 Jul;37(7):970-2. PMID: 17581190
MH  - Anti-Allergic Agents/*pharmacology/therapeutic use
MH  - Anti-Asthmatic Agents/*pharmacology/therapeutic use
MH  - Antigens, CD/metabolism
MH  - Arylsulfonates/*pharmacology/therapeutic use
MH  - Asthma/*drug therapy/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Case-Control Studies
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL17
MH  - Chemokines, CC/metabolism
MH  - Coculture Techniques
MH  - Dendritic Cells/*drug effects/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-12 Subunit p40/metabolism
MH  - Interleukin-4/metabolism
MH  - Interleukin-5/metabolism
MH  - Lymphocyte Activation
MH  - Monocytes/*drug effects/immunology
MH  - Sulfonium Compounds/*pharmacology/therapeutic use
EDAT- 2007/06/22 09:00
MHDA- 2007/10/12 09:00
CRDT- 2007/06/22 09:00
PHST- 2007/06/22 09:00 [pubmed]
PHST- 2007/10/12 09:00 [medline]
PHST- 2007/06/22 09:00 [entrez]
AID - CEA2616 [pii]
AID - 10.1111/j.1365-2222.2006.02616.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2007 Jul;37(7):1083-9. doi: 10.1111/j.1365-2222.2006.02616.x.