PMID- 17585182
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20190608
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 53
IP  - 5
DP  - 2007 Oct
TI  - Immunolocalization of steroidogenic enzymes in equine fetal adrenal glands during 
      mid-late gestation.
PG  - 1093-8
AB  - To elucidate the relationship between steroidogenic hormones and developing 
      adrenal glands, we investigated the immunolocalization of steroidogenic enzymes 
      in equine fetal adrenal glands during mid-late gestation. Fetal adrenal glands 
      were obtained from three horses at 217, 225 and 235 days of gestation. 
      Steroidogenic enzymes were immunolocalized using polyclonal antisera raised 
      against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), 
      human placental 3beta-hydroxysteroid dehydrogenase (3betaHSD), porcine testicular 
      17alpha-hydroxylase cytochrome P450 (P450c17) and human placental aromatase 
      cytochrome P450 (P450arom). Histologically, cortex and medulla cells were clearly 
      observed in the three fetal adrenal gland tissue samples. P450scc and P450c17 
      were identified in cortex cells close to medulla cells and in some medulla cells 
      in the fetal adrenal glands. P450arom was present in both cortex and medulla 
      cells in the fetal adrenal glands. However, 3betaHSD was not found in any of the 
      equine fetal adrenal gland tissue samples. These results suggest that equine 
      fetal adrenal glands have the ability to synthesize androgen and estrogen, which 
      may play an important physiological role in the development of equine fetal 
      adrenal glands.
FAU - Weng, Qiang
AU  - Weng Q
AD  - College of Biological Science and Technology, Beijing Forestry University, 
      Beijing, P.R. China. weng3@msn.com
FAU - Tanaka, Yumiko
AU  - Tanaka Y
FAU - Taniyama, Hiroyuki
AU  - Taniyama H
FAU - Tsunoda, Nobuo
AU  - Tsunoda N
FAU - Nambo, Yasuo
AU  - Nambo Y
FAU - Watanabe, Gen
AU  - Watanabe G
FAU - Taya, Kazuyoshi
AU  - Taya K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070622
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Androgens)
RN  - 0 (Estrogens)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.- (Steroid Hydroxylases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/analysis
MH  - Adrenal Glands/*embryology/*enzymology
MH  - Androgens/biosynthesis
MH  - Animals
MH  - Cattle
MH  - Estrogens/biosynthesis
MH  - Female
MH  - Fetus/*enzymology
MH  - Horses/*embryology
MH  - Humans
MH  - Immunohistochemistry
MH  - Pregnancy
MH  - Steroid Hydroxylases/*analysis
EDAT- 2007/06/23 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/06/23 09:00
PHST- 2007/06/23 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/06/23 09:00 [entrez]
AID - JST.JSTAGE/jrd/18159 [pii]
AID - 10.1262/jrd.18159 [doi]
PST - ppublish
SO  - J Reprod Dev. 2007 Oct;53(5):1093-8. doi: 10.1262/jrd.18159. Epub 2007 Jun 22.