PMID- 17587448
OWN - NLM
STAT- MEDLINE
DCOM- 20070808
LR  - 20181113
IS  - 1471-2199 (Electronic)
IS  - 1471-2199 (Linking)
VI  - 8
DP  - 2007 Jun 22
TI  - Structural and RNAi characterization of the German cockroach lipophorin receptor, 
      and the evolutionary relationships of lipoprotein receptors.
PG  - 53
AB  - BACKGROUND: Lipophorin receptors (LpRs) have been described in a number of 
      insects, but functional studies have been reported only in locusts and 
      mosquitoes. The aim of the present work was to characterize the LpR of the 
      cockroach Blattella germanica, not only molecularly but also functionally using 
      RNAi techniques, and to place LpRs in a phylogenetical context among lipoprotein 
      receptors. RESULTS: We cloned a putative LpR from B. germanica (BgLpR) using 
      RT-PCR methods. Two isoforms of BgLpR that differ from each other by an 
      insertion/deletion of 24 amino acids were obtained from the fat body and the 
      ovary. A phylogenetical analysis of lipoprotein receptors showed that BgLpR 
      grouped with other sequences annotated as LpR in a cluster placed as a sister 
      group of vertebrate low density lipoprotein receptors (LDLR) + lipoprotein 
      receptor-related proteins 8 (LPR8) + vitellogenin receptors (VgR) + very low 
      density lipoprotein receptors (VLDLR). The two BgLpR isoforms are expressed in 
      different adult female tissues (fat body, ovary, brain, midgut, muscle) and in 
      embryos. In ovaries and fat body, the two isoforms are similarly expressed during 
      the first gonadotrophic cycle. mRNA levels in the fat body increase in parallel 
      to vitellogenesis, whereas they decrease in the ovaries. BgLpR protein levels 
      increase in parallel to vitellogenesis in both organs. Treatment with juvenile 
      hormone increases BgLpR protein. RNAi experiments show that females with lower 
      BgLpR expression have less lipophorin in the growing oocytes with respect to 
      controls. CONCLUSION: The two isoforms of BgLpR are structurally similar to other 
      LpRs. Phylogenetical analyses show that LpRs and the group formed by vertebrate 
      LDLR + LPR8 + VgR + VLDLR, diverged from a common ancestor and diversified in 
      parallel. The different expression patterns in the fat body and the ovary, 
      comparing mRNA and protein, indicate that the corresponding mechanisms regulating 
      BgLpR expression are different. Experiments with JH III suggest that such a 
      hormone regulates the expression of BgLpR posttranscriptionally. RNAi experiments 
      indicate that BgLpR is a functional lipophorin receptor.
FAU - Ciudad, Laura
AU  - Ciudad L
AD  - Department of Physiology and Molecular Biodiversity, Institut de Biologia 
      Molecular de Barcelona, CSIC, Jordi Girona 18, Barcelona, Spain. 
      lcgagr@cid.csic.es <lcgagr@cid.csic.es>
FAU - Belles, Xavier
AU  - Belles X
FAU - Piulachs, Maria-Dolors
AU  - Piulachs MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070622
PL  - England
TA  - BMC Mol Biol
JT  - BMC molecular biology
JID - 100966983
RN  - 0 (Insect Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (lipophorin receptor)
SB  - IM
MH  - Animals
MH  - Cockroaches
MH  - Evolution, Molecular
MH  - Female
MH  - Gene Expression
MH  - Insect Proteins/*genetics
MH  - Ovary/metabolism
MH  - Phylogeny
MH  - RNA Interference
MH  - Receptors, Cytoplasmic and Nuclear/*genetics
MH  - Sequence Homology, Amino Acid
PMC - PMC1933434
EDAT- 2007/06/26 09:00
MHDA- 2007/08/09 09:00
PMCR- 2007/06/22
CRDT- 2007/06/26 09:00
PHST- 2007/01/15 00:00 [received]
PHST- 2007/06/22 00:00 [accepted]
PHST- 2007/06/26 09:00 [pubmed]
PHST- 2007/08/09 09:00 [medline]
PHST- 2007/06/26 09:00 [entrez]
PHST- 2007/06/22 00:00 [pmc-release]
AID - 1471-2199-8-53 [pii]
AID - 10.1186/1471-2199-8-53 [doi]
PST - epublish
SO  - BMC Mol Biol. 2007 Jun 22;8:53. doi: 10.1186/1471-2199-8-53.