PMID- 17587761
OWN - NLM
STAT- MEDLINE
DCOM- 20070822
LR  - 20190608
IS  - 1340-3478 (Print)
IS  - 1340-3478 (Linking)
VI  - 14
IP  - 3
DP  - 2007 Jun
TI  - Role of Ca(2+)influx in tissue factor expression in monocyte adhesion to 
      endothelial cells.
PG  - 109-15
AB  - Tissue factor (TF) is the primary initiator of the coagulation cascade. Ca(2+) 
      signaling is involved in TF gene expression. Monocyte chemoattractant protein-1 
      (MCP-1) and its receptor (CCR2) play a pivotal role in the inflammation of 
      atherosclerosis. Although nitric oxide (NO) impairment appears to promote 
      thrombogenicity in monocyte adhesion to endothelial cells (ECs), little is known 
      about its mechanism. N(omega)-nitro-L-arginine methyl ester (L-NAME) promoted 
      MCP-1 expression in EC culture. In response to monocyte adhesion, increased TF 
      expression accompanied by NF-kappaB p65 activation was observed in L-NAME-treated 
      ECs compared with non-treated ECs. This increased TF expression was prevented by 
      BAPTA-AM, an intracellular Ca(2+) chelator. Monocyte attachment to L-NAME- 
      treated ECs increased Ca(2+) influx compared with non-treated ECs, which was 
      prevented by the blockade of MCP1/CCR2. These findings suggest that increased 
      production of MCP-1 caused by L-NAME contributes to the enhancement of Ca(2+) 
      influx only when monocytes adhered to ECs and that this may accelerate TF 
      expression in ECs triggered by monocyte adhesion. We demonstrate the role of 
      Ca(2+) influx via MCP-1/CCR2 under NO impairment in TF expression in monocyte-EC 
      interaction.
FAU - Sakamoto, Takayuki
AU  - Sakamoto T
AD  - First Department of Internal Medicine, Fukushima Medical University, Hikarigaoka, 
      Fukushima, Japan. sakamoto@fmu.ac.jp
FAU - Ishibashi, Toshiyuki
AU  - Ishibashi T
FAU - Maruyama, Yukio
AU  - Maruyama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Atheroscler Thromb
JT  - Journal of atherosclerosis and thrombosis
JID - 9506298
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9035-58-9 (Thromboplastin)
RN  - SY7Q814VUP (Calcium)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Calcium/*metabolism
MH  - Calcium Signaling/physiology
MH  - *Cell Adhesion
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Inflammation
MH  - Monocytes/*metabolism
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/antagonists & inhibitors/metabolism
MH  - Thromboplastin/*metabolism
EDAT- 2007/06/26 09:00
MHDA- 2007/08/23 09:00
CRDT- 2007/06/26 09:00
PHST- 2007/06/26 09:00 [pubmed]
PHST- 2007/08/23 09:00 [medline]
PHST- 2007/06/26 09:00 [entrez]
AID - JST.JSTAGE/jat/14.109 [pii]
AID - 10.5551/jat.14.109 [doi]
PST - ppublish
SO  - J Atheroscler Thromb. 2007 Jun;14(3):109-15. doi: 10.5551/jat.14.109.