PMID- 17589298
OWN - NLM
STAT- MEDLINE
DCOM- 20070817
LR  - 20080318
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 30
IP  - 5
DP  - 2007 Jul-Aug
TI  - A comparative analysis of serum and serum-free media for generation of clinical 
      grade DCs.
PG  - 567-76
AB  - Dendritic cells (DCs) are the most potent antigen presenting cells and are 
      therefore widely used in cancer immunotherapy. An optimal method for the 
      generation of DCs for clinical use remains to be established. The aim of the 
      study was to find a serum-free media (SFM) able to generate reproducible and 
      functional cultures of DCs for clinical studies. We characterized immature and 
      mature DCs cultured in SFM, CellGro DC and X-VIVO15, and serum media (SM), RPMI 
      1640+5% human serum or autologous serum. The expression of HLA-DR, CD86, CD83 was 
      higher in SM-cultured DCs (SM-DCs) than SFM-derived DCs (SFM-DCs). Between 
      SFM-DCs, CellGro-cultured DCs (CellGro-DCs) showed a higher expression and an 
      improved up-regulation capacity of all molecules as compared with 
      X-VIVO15-derived DCs (X-VIVO15-DCs). CellGro-DCs and SM-DCs showed a similar 
      mannose receptor expression and related endocytic capacity tested by fluorescein 
      isothiocyanate-dextran uptake. In contrast X-VIVO15-DCs expressed low levels of 
      mannose receptor and were unable to endocyte fluorescein isothiocyanate-dextran. 
      DCs cultured in all conditions stimulated a mix lymphocyte reaction, but 
      CellGro-DCs and SM-DCs induced a more potent T-cell proliferation compared with 
      X-VIVO15-DCs. Cytokine analysis showed that after maturation, all DC cultures 
      produced IL-12p70 and IL-10 except for X-VIVO15-DCs which only produced the 
      latter cytokine. SM-DCs and SFM-DCs induced a TH1 polarization in allogeneic 
      naive T cells. In conclusion, a comparative analysis of DC performance generated 
      in different conditions allows us to determine CellGro DC as the optimal medium 
      for the generation of clinical grade DCs.
FAU - Napoletano, Chiara
AU  - Napoletano C
AD  - Department of Experimental Medicine, University of Rome La Sapienza, Viale Regina 
      Elena 324, 00161 Rome, Italy.
FAU - Pinto, Dora
AU  - Pinto D
FAU - Bellati, Filippo
AU  - Bellati F
FAU - Taurino, Federica
AU  - Taurino F
FAU - Rahimi, Hassan
AU  - Rahimi H
FAU - Tomao, Federica
AU  - Tomao F
FAU - Panici, Pierluigi Benedetti
AU  - Panici PB
FAU - Rughetti, Aurelia
AU  - Rughetti A
FAU - Frati, Luigi
AU  - Frati L
FAU - Nuti, Marianna
AU  - Nuti M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Culture Media)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Cytokines)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - *Culture Media
MH  - Culture Media, Serum-Free
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Endocytosis
MH  - Humans
MH  - Immunophenotyping
MH  - Th1 Cells/cytology/immunology/metabolism
MH  - Th2 Cells/cytology/immunology/metabolism
EDAT- 2007/06/26 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/06/26 09:00
PHST- 2007/06/26 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/06/26 09:00 [entrez]
AID - 00002371-200707000-00012 [pii]
AID - 10.1097/CJI.0b013e318046f396 [doi]
PST - ppublish
SO  - J Immunother. 2007 Jul-Aug;30(5):567-76. doi: 10.1097/CJI.0b013e318046f396.