PMID- 17589922
OWN - NLM
STAT- MEDLINE
DCOM- 20070912
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 13
IP  - 22
DP  - 2007 Jun 14
TI  - Overexpression of the cholesterol-binding protein MLN64 induces liver damage in 
      the mouse.
PG  - 3071-9
AB  - AIM: To examine the in vivo phenotype associated with hepatic metastatic lymph 
      node 64 (MLN64) over-expression. METHODS: Recombinant-adenovirus-mediated MLN64 
      gene transfer was used to overexpress MLN64 in the livers of C57BL/6 mice. We 
      measured the effects of MLN64 overexpression on hepatic cholesterol content, bile 
      flow, biliary lipid secretion and apoptosis markers. For in vitro studies 
      cultured CHO cells with transient MLN64 overexpression were utilized and 
      apoptosis by TUNEL assay was measured. RESULTS: Livers from Ad.MLN64-infected 
      mice exhibited early onset of liver damage and apoptosis. This response 
      correlated with increases in liver cholesterol content and biliary bile acid 
      concentration, and impaired bile flow. We investigated whether liver MLN64 
      expression could be modulated in a murine model of hepatic injury. We found 
      increased hepatic MLN64 mRNA and protein levels in mice with chenodeoxycholic 
      acid-induced liver damage. In addition, cultured CHO cells with transient MLN64 
      overexpression showed increased apoptosis. CONCLUSION: In summary, hepatic MLN64 
      over-expression induced damage and apoptosis in murine livers and altered 
      cholesterol metabolism. Further studies are required to elucidate the relevance 
      of these findings under physiologic and disease conditions.
FAU - Tichauer, Juan-Enrique
AU  - Tichauer JE
AD  - Departamento de Gastroenterologia, Pontificia Universidad Catolica de Chile, 
      Marcoleta 367, Santiago, Chile.
FAU - Morales, Maria-Gabriela
AU  - Morales MG
FAU - Amigo, Ludwig
AU  - Amigo L
FAU - Galdames, Leopoldo
AU  - Galdames L
FAU - Klein, Andres
AU  - Klein A
FAU - Quinones, Veronica
AU  - Quinones V
FAU - Ferrada, Carla
AU  - Ferrada C
FAU - Alvarez, Alejandra-R
AU  - Alvarez AR
FAU - Rio, Marie-Christine
AU  - Rio MC
FAU - Miquel, Juan-Francisco
AU  - Miquel JF
FAU - Rigotti, Attilio
AU  - Rigotti A
FAU - Zanlungo, Silvana
AU  - Zanlungo S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (MLN64 protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0GEI24LG0J (Chenodeoxycholic Acid)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Alkaline Phosphatase/blood
MH  - Animals
MH  - *Apoptosis
MH  - CHO Cells
MH  - Cell Line
MH  - Chemical and Drug Induced Liver Injury
MH  - Chenodeoxycholic Acid
MH  - Cholesterol/metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - Humans
MH  - Liver/metabolism/pathology
MH  - Liver Diseases/*metabolism/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Phosphoproteins/genetics/*metabolism
MH  - Transfection
PMC - PMC4172613
EDAT- 2007/06/26 09:00
MHDA- 2007/09/13 09:00
PMCR- 2007/06/14
CRDT- 2007/06/26 09:00
PHST- 2007/06/26 09:00 [pubmed]
PHST- 2007/09/13 09:00 [medline]
PHST- 2007/06/26 09:00 [entrez]
PHST- 2007/06/14 00:00 [pmc-release]
AID - 10.3748/wjg.v13.i22.3071 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2007 Jun 14;13(22):3071-9. doi: 10.3748/wjg.v13.i22.3071.