PMID- 17591268
OWN - NLM
STAT- MEDLINE
DCOM- 20090521
LR  - 20181201
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 24
IP  - 2
DP  - 2007 Apr
TI  - [Tissue engineering peripheral nerve with TGF-beta repair sciatic nerve defect].
PG  - 394-8
AB  - The purpose of this investigation was to evaluate the effect of transforming 
      growth factor-beta (TGF-beta) on the efficacy of Schwann cell (SC) and on the 
      repair of peripheral nerve defect. 50 ng x ml(-1) TGF-beta was shown to promote 
      the proliferation of SC by MTT and flow cytometry (FCM) assay, and NGF synthesis 
      in SC culture media was noted to be of significantly higher concentration by 
      ELISA method (P<0.05). SCs mixed with bovine acellular matrix (BAM), fetal bovine 
      serum and media based on definite ratio were injected into 
      polylactideco-glycolide acid (PLGA)guide. 30 SD rats, each had a man-made sciatic 
      nerve defect 15 mm long, were randomly divided into 3 groups: experiment group 
      (PLGA conduit+SC+TGF-beta), control group(PLGA conduit+SC), and autograft group. 
      After 16 weeks, it was demonstrated that the effect of the test group was not 
      significantly different from that of the autograft group, but it was better than 
      that of the control group by means of electrophysiological test and sciatic nerve 
      function index (SFI). TGF-beta can promote not only the proliferation, but also 
      the NGF synthesis of SC obviously. The use of exogenous TGF-beta in the repair of 
      peripheral nerve defect may produce better curative effect.
FAU - Zhang, Yongjie
AU  - Zhang Y
AD  - Tissue Engineering Center,Department of Oral Histology & Pathology, College of 
      Stomatology, The Fourth Military Medical University, Xi'an, China.
FAU - Jin, Yan
AU  - Jin Y
FAU - Nie, Xin
AU  - Nie X
FAU - Wang, Yanliang
AU  - Wang Y
FAU - Liu, Peng
AU  - Liu P
FAU - Shen, Nan
AU  - Shen N
FAU - Guo, Shenghui
AU  - Guo S
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Culture Media)
RN  - 0 (Transforming Growth Factor beta)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Culture Media
MH  - Female
MH  - *Guided Tissue Regeneration
MH  - Lactic Acid/chemistry
MH  - Nerve Growth Factor/biosynthesis
MH  - Nerve Regeneration/*drug effects
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/cytology
MH  - Sciatic Nerve/*injuries/physiology
MH  - *Tissue Engineering
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2007/06/27 09:00
MHDA- 2009/05/22 09:00
CRDT- 2007/06/27 09:00
PHST- 2007/06/27 09:00 [pubmed]
PHST- 2009/05/22 09:00 [medline]
PHST- 2007/06/27 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2007 Apr;24(2):394-8.