PMID- 17592021
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20161124
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 194
IP  - 1
DP  - 2007 Jul
TI  - Increased metastatic lymph node 64 and CYP17 expression are associated with high 
      stage prostate cancer.
PG  - 55-61
AB  - The metastatic lymph node 64 (MLN64), which is localized in the human chromosome 
      17, encodes a protein with strong homology with steroidogenic acute regulatory 
      protein. Its overexpression in human breast carcinomas and MLNs led to the 
      hypothesis that this protein could be involved in intraneoplastic 
      steroidogenesis. In the present study, we investigated the expression of MLN64 in 
      prostate cancer, another hormone-dependent tumor, and compared its expression 
      with that of CYP17, the gene encoding for the key enzyme of androgen synthesis. 
      We investigated by RT-PCR the expression of MLN64 and CYP17 in 60 prostatic 
      tumors and compared their expression with the stage of disease and the appearance 
      of relapses in a follow-up of 24 months. We found MLN64 and CYP17 expressed in 
      all samples examined, with significantly higher expression in neoplastic tissues 
      with respect to normal tissues (NTs). Moreover, only in neoplastic but not in 
      NTs, a positive linear correlation was found between MLN64 and CYP17 gene 
      expression. MLN64 and CYP17 expression seems to correlate with high stage, high 
      Gleason score and short relapse-free time. These data, for the first time, 
      demonstrate the presence of MLN64 and CYP17 expression in both normal and 
      neoplastic prostatic tissues. The biological role of MLN64 in human prostate and, 
      particularly, in neoplastic tissue is still unclear. Our findings concerning 
      MLN64 and CYP17 gene expression and their significant positive correlation in 
      human prostate cancer may suggest their possible role in intraneoplastic 
      autonomous steroidogenesis.
FAU - Stigliano, A
AU  - Stigliano A
AD  - Endocrinology II Faculty of Medicine, University of Rome La Sapienza, Via Cassia, 
      600-00189 Rome, Italy. antonio.stigliano@uniromal.it
FAU - Gandini, O
AU  - Gandini O
FAU - Cerquetti, L
AU  - Cerquetti L
FAU - Gazzaniga, P
AU  - Gazzaniga P
FAU - Misiti, S
AU  - Misiti S
FAU - Monti, S
AU  - Monti S
FAU - Gradilone, A
AU  - Gradilone A
FAU - Falasca, P
AU  - Falasca P
FAU - Poggi, M
AU  - Poggi M
FAU - Brunetti, E
AU  - Brunetti E
FAU - Agliano, A M
AU  - Agliano AM
FAU - Toscano, V
AU  - Toscano V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Androgens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (STARD3 protein, human)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
EIN - J Endocrinol. 2007 Aug;194(2):458
MH  - Aged
MH  - Androgens/biosynthesis
MH  - Blotting, Western/methods
MH  - Carrier Proteins/*genetics/metabolism
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Membrane Proteins/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/*enzymology/pathology
MH  - Neoplasm Staging
MH  - Probability
MH  - Prostatic Neoplasms/*enzymology/pathology
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Steroid 17-alpha-Hydroxylase/*genetics/metabolism
EDAT- 2007/06/27 09:00
MHDA- 2007/09/18 09:00
CRDT- 2007/06/27 09:00
PHST- 2007/06/27 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2007/06/27 09:00 [entrez]
AID - 194/1/55 [pii]
AID - 10.1677/JOE-07-0131 [doi]
PST - ppublish
SO  - J Endocrinol. 2007 Jul;194(1):55-61. doi: 10.1677/JOE-07-0131.