PMID- 17592529
OWN - NLM
STAT- MEDLINE
DCOM- 20070809
LR  - 20240326
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Electronic)
IS  - 1995-8218 (Linking)
VI  - 23
IP  - 2
DP  - 2007 Mar
TI  - Expression of motilin in the hypothalamus and the effect of central erythromycin 
      on gastric motility in diabetic rats.
PG  - 75-82
AB  - OBJECTIVE: To investigate the expression of motilin-immunoreactive neurons in the 
      hypothalamus and the effect of central administration of erythromycin (EM) on the 
      regulation of gastric motility in diabetic rats. METHODS: The motilin 
      immunoreactive neurons in the hypothalamus and the hippocampus were detected by 
      immunohistochemistry with rabbit anti-motilin polyclonal antibody. To measure the 
      gastric motility, force transducers were surgically affixed to the gastric 
      serosa. A microinjection syringe was connected via a plastic tube to an injection 
      cannula, which was connected with a stainless steel guide cannula. The syringe 
      was inserted into the right lateral cerebral ventricle for microinjecting the 
      chemicals. RESULTS: Diabetic mellitus was successfully induced in cohorts of 
      rats. Motilin-immunoreactive neurons significantly increased in the 
      paraventricular (PVN) and supraoptic nuclei (SON) of the hypothalamus in the 
      diabetic rats. Intracerebroventricular (i.c.v.) administration of EM, a motilin 
      receptor agonist, stimulated the gastric motility of diabetic rats. EM (91.56 
      nmol, i.c.v.) dose-dependently increased the amplitude by (174.82 +/- 48.62)% 
      (P<0.05), and increased the frequency by (70.43 +/- 27.11)% (P < 0.05) in 5 min. 
      The stimulatory effect lasted more than 15 min to the end of the measurement, and 
      can be blocked partially by the prior treatment of motilin receptor antagonist 
      GM-109. CONCLUSION: Motilin-immunoreactive neurons are increased in the PVN and 
      SON of the hypothalamus in diabetic rats. Centrally administered EM may regulate 
      gastric motility by binding to the central motilin receptors, and central motilin 
      might be involved in regulation of gastric motility in diabetic rats.
FAU - Jia, Yun-Dan
AU  - Jia YD
AD  - Department of Physiology, School of Medicine, Qingdao University, Qingdao 266071, 
      China.
FAU - Liu, Chang-Qin
AU  - Liu CQ
FAU - Tang, Ming
AU  - Tang M
FAU - Jiang, Zheng-Yao
AU  - Jiang ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Receptors, Gastrointestinal Hormone)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (motilin receptor)
RN  - 52906-92-0 (Motilin)
RN  - 63937KV33D (Erythromycin)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Erythromycin/administration & dosage/*pharmacology
MH  - Gastrointestinal Agents/administration & dosage/*pharmacology
MH  - Gastrointestinal Motility/drug effects/*physiology
MH  - Hippocampus/cytology/metabolism
MH  - Injections, Intraventricular
MH  - Male
MH  - Microinjections
MH  - Motilin/agonists/*metabolism
MH  - Neurons/cytology/metabolism
MH  - Paraventricular Hypothalamic Nucleus/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Gastrointestinal Hormone/agonists
MH  - Receptors, Neuropeptide/agonists
MH  - Statistics, Nonparametric
MH  - Supraoptic Nucleus/cytology/*metabolism
PMC - PMC5550590
EDAT- 2007/06/27 09:00
MHDA- 2007/08/10 09:00
PMCR- 2008/03/01
CRDT- 2007/06/27 09:00
PHST- 2007/06/27 09:00 [pubmed]
PHST- 2007/08/10 09:00 [medline]
PHST- 2007/06/27 09:00 [entrez]
PHST- 2008/03/01 00:00 [pmc-release]
AID - 11 [pii]
AID - 10.1007/s12264-007-0011-4 [doi]
PST - ppublish
SO  - Neurosci Bull. 2007 Mar;23(2):75-82. doi: 10.1007/s12264-007-0011-4.