PMID- 17593080
OWN - NLM
STAT- MEDLINE
DCOM- 20070920
LR  - 20141120
IS  - 0309-0167 (Print)
IS  - 0309-0167 (Linking)
VI  - 51
IP  - 1
DP  - 2007 Jul
TI  - Differential expression of c-Met, its ligand HGF/SF and HER2/neu in DCIS and 
      adjacent normal breast tissue.
PG  - 54-62
AB  - AIMS: Tyrosine kinase receptors Her2/neu and c-Met play an important role in 
      breast cancer development and progression. Our aim was to determine the 
      expression of c-Met, its ligand hepatocyte growth factor/scatter factor (HGF/SF) 
      and Her2/neu in ductal carcinoma in situ (DCIS) lesions of the breast (n = 39) by 
      two different immunocytochemical techniques, classical immunohistochemistry and 
      immunofluorescence, and to correlate their expression levels with 
      histopathological and clinical characteristics. METHODS AND RESULTS: Both methods 
      revealed similar c-Met staining patterns in both the in situ component and the 
      adjacent normal tissue (P < 0.001). However, an imbalance in c-Met expression 
      between tumour and surrounding normal tissue was correlated with high-grade DCIS 
      (Van Nuys Grade 3). No correlation existed between Her2/neu and c-Met expression. 
      High HGF/SF immunoreactivity was observed in 43.6% of the cases, yet the adjacent 
      cellular stroma revealed only low levels of HGF/SF. No correlation existed 
      between c-Met, Her2/neu or HGF/SF expression and clinicopathological factors. 
      CONCLUSION: An imbalance in c-Met expression between tumour and surrounding 
      normal tissue is associated with an aggressive DCIS phenotype. Moreover, c-Met 
      and HGF/SF may contribute to tumour development by different means than those 
      controlled by Her2/neu.
FAU - Lindemann, K
AU  - Lindemann K
AD  - Department of Obstetrics and Gynaecology, Technical University, Munich, Germany.
FAU - Resau, J
AU  - Resau J
FAU - Nahrig, J
AU  - Nahrig J
FAU - Kort, E
AU  - Kort E
FAU - Leeser, B
AU  - Leeser B
FAU - Annecke, K
AU  - Annecke K
FAU - Welk, A
AU  - Welk A
FAU - Schafer, J
AU  - Schafer J
FAU - Vande Woude, G F
AU  - Vande Woude GF
FAU - Lengyel, E
AU  - Lengyel E
FAU - Harbeck, N
AU  - Harbeck N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast/cytology/*metabolism
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/*metabolism/pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-met/genetics/*metabolism
MH  - Receptor, ErbB-2/genetics/*metabolism
EDAT- 2007/06/27 09:00
MHDA- 2007/09/21 09:00
CRDT- 2007/06/27 09:00
PHST- 2007/06/27 09:00 [pubmed]
PHST- 2007/09/21 09:00 [medline]
PHST- 2007/06/27 09:00 [entrez]
AID - HIS2732 [pii]
AID - 10.1111/j.1365-2559.2007.02732.x [doi]
PST - ppublish
SO  - Histopathology. 2007 Jul;51(1):54-62. doi: 10.1111/j.1365-2559.2007.02732.x.