PMID- 17593139
OWN - NLM
STAT- MEDLINE
DCOM- 20070827
LR  - 20151119
IS  - 1350-1925 (Print)
IS  - 1350-1925 (Linking)
VI  - 19
IP  - 7
DP  - 2007 Jul
TI  - Chromosomal study of enteric glial cells and neurons by fluorescence in situ 
      hybridization in slow transit constipation.
PG  - 578-84
AB  - The pathogenesis of slow transit constipation is still elusive. However, a 
      genetic basis may be present. We investigated possible chromosomal abnormalities 
      in enteric neurons and glial cells in patients with slow transit constipation. 
      Colonic specimens from 22 patients with slow transit constipation undergoing 
      surgery for intractable symptoms were obtained, and investigated by fluorescence 
      in situ hybridization (FISH) for chromosomal abnormalities (chromosomes 1, 8, 17 
      and XY). These specimens were compared with of those obtained in 12 control 
      subjects. Data analysis showed that 45.5% of patients displayed significant 
      (>10%) aneusomy of chromosome 1 in enteric neurons. Aneusomy <10% for the same 
      chromosome, but less than the cutoff suggested (10%), was found in enteric glial 
      cells in 45.4% of the same patients. One patient had <10% aneusomy in enteric 
      neurons for chromosome 8. No other abnormalities were found for the remaining 
      probes, and no abnormalities were found in controls. We concluded that in a 
      subgroup of patients with slow transit constipation a genetic basis may be 
      present.
FAU - Rossi, E
AU  - Rossi E
AD  - Second Department of Pathology, Spedali Civili, Brescia, Italy.
FAU - Villanacci, V
AU  - Villanacci V
FAU - Fisogni, S
AU  - Fisogni S
FAU - Morelli, A
AU  - Morelli A
FAU - Salerni, B
AU  - Salerni B
FAU - Grigolato, P
AU  - Grigolato P
FAU - Bassotti, G
AU  - Bassotti G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers
MH  - Biopsy
MH  - *Chromosome Aberrations
MH  - Constipation/*genetics/*pathology/physiopathology
MH  - Enteric Nervous System/*pathology/physiology
MH  - Female
MH  - Gastrointestinal Transit
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neuroglia/pathology/physiology
MH  - Neurons/pathology/physiology
EDAT- 2007/06/27 09:00
MHDA- 2007/08/28 09:00
CRDT- 2007/06/27 09:00
PHST- 2007/06/27 09:00 [pubmed]
PHST- 2007/08/28 09:00 [medline]
PHST- 2007/06/27 09:00 [entrez]
AID - NMO914 [pii]
AID - 10.1111/j.1365-2982.2007.00914.x [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2007 Jul;19(7):578-84. doi: 
      10.1111/j.1365-2982.2007.00914.x.