PMID- 17594282
OWN - NLM
STAT- MEDLINE
DCOM- 20081124
LR  - 20181113
IS  - 1729-0503 (Electronic)
IS  - 1680-6905 (Print)
IS  - 1680-6905 (Linking)
VI  - 7
IP  - 2
DP  - 2007 Jun
TI  - Organisms associated with bacterial vaginosis in Nigerian women as determined by 
      PCR-DGGE and 16S rRNA gene sequence.
PG  - 68-72
AB  - BACKGROUND: Bacterial vaginosis (BV) is a condition with diverse etiology. This 
      condition predisposes women to increased susceptibility to sexually transmitted 
      diseases, including human immunodeficiency virus (HIV) infections and preterm 
      birth. The diagnostic methods currently adopted in the evaluation of patient 
      samples for BV are arguably Amsel criteria, and Nugent score that require 
      microscopy and expert interpretation. These two methods are still subjective. 
      OBJECTIVE: The objective of this study was to determine the organisms present in 
      the vagina of 34 HIV negative Nigerian women diagnosed as having bacterial 
      vaginosis by using molecular techniques. METHODS: The vaginal samples were 
      subjected to DNA extraction, and amplified with eubacterial primers via PCR. The 
      PCR products were separated using denaturing gradient gel electrophoresis (DGGE). 
      Bands were excised, re-amplified, purified and sequenced. Sequence identification 
      was performed using the BLAST algorithm and Genbank data base. RESULTS: 
      Mycoplasma hominis (12/34; 35%) was the most common isolate and 9 (26%) contained 
      one of two clones of an unusual Rainbow Trout intestinal bacterium, while 
      unculturable Streptococcus sp, and other bacteria made up the remaining isolates. 
      CONCLUSIONS: The findings indicate further diversity in the etiological agents 
      associated with BV, and raise the question as to whether diagnosis and management 
      of this condition needs to be re-evaluated in countries like Nigeria. There is 
      some controversy over the clinical importance of BV, as it was once regarded as a 
      disease caused by Gardnerella and presenting as an odourous discharge condition, 
      but is now diagnosed without necessarily the presence of these organisms or 
      signs. With the incidence of BV aligned to an increased risk of HIV in a country 
      ravaged by this virus, the effective eradication of BV can only be achieved if 
      appropriate therapies are delivered.
FAU - Anukam, Kingsley C
AU  - Anukam KC
AD  - Canadian Research and Development Centre for Probiotic, Lawson Health Research 
      Institute, 268 Grosvenor Street, London, Ontario, Canada. kanukam@uwo.ca
FAU - Reid, Gregor
AU  - Reid G
LA  - eng
PT  - Journal Article
PL  - Uganda
TA  - Afr Health Sci
JT  - African health sciences
JID - 101149451
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - DNA, Bacterial/analysis
MH  - Electrophoresis, Agar Gel/*methods
MH  - Female
MH  - Humans
MH  - *Molecular Sequence Data
MH  - Mycoplasma hominis/pathogenicity
MH  - Nigeria
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Vaginosis, Bacterial/diagnosis/*etiology/microbiology
PMC - PMC1925267
EDAT- 2007/06/28 09:00
MHDA- 2008/12/17 09:00
PMCR- 2007/06/01
CRDT- 2007/06/28 09:00
PHST- 2007/06/28 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2007/06/28 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - jAFHS.v7.i2.pg68 [pii]
AID - 10.5555/afhs.2007.7.2.68 [doi]
PST - ppublish
SO  - Afr Health Sci. 2007 Jun;7(2):68-72. doi: 10.5555/afhs.2007.7.2.68.